典型文献
Systematic mutational analysis of epitope-grafted ED3 immunogenicity reveals a DENV3-DENV4 bi-serospecific ED3 mutant
文献摘要：
Objective:To identify the residue determinants of the serospecificity and sero-cross-reactivity of dengue virus(DENV)envelope protein domain 3(ED3),which contains two major putative epitopes of DENV.Methods:We constructed ED3 from DENV3(3ED3)and DENV4(4ED3),and six epitope-grafted variants,where we transferred epitope 1(L304I,K305D,V309M,and S310A)and/or epitope 2(D383N,K384S,K387T,and N389H)of 4ED3 onto 3ED3 and vice-versa.Swiss albino mice aged 3-4 weeks were immunized against wildtype and epitope-grafted ED3 variants and anti-ED3 IgG antibody responses were determined using ELISA.Results:Mouse immunization using 3ED3 and 4ED3 generated serotype-specific antisera,as expected.Similarly,most epitope-grafted ED3s produced antisera serospecific to the template ED3 with little or no cross-recognition of ED3 of the serotype from which the epitopes were taken.These indicated that a mere grafting of the epitope was not sufficient to transfer serospecificity,contrary to our expectations.However,one epitope-grafted ED3 mutant,where epitope 1 of 3ED3 was grafted onto 4ED3(4ED3epi1),generated antisera that was serospecific to both 4ED3 and 3ED3.Conclusions:The 4ED3epi1 is a chimeric ED3 that produces antisera possessing serospecificity to both 3ED3 and 4ED3 onto a common 4ED3 scaffold.The 4ED3epi1,therefore,provides a unique tool for analyzing serospecificity and sero-cross-reactivity in dengue.We believe that chimeric ED3 may provide a template for future recombinant ED3 possessing serospecificity of multiple DENVs onto a single scaffold and may pave a way developing tri-and/or tetravalent anti-DENV antisera.
文献关键词：
中图分类号：
作者姓名：
Mamtaz Sultana;Nazmul Hasan;Mamunur R.Mahib;Mohammad M.Islam
作者机构：
Department of Biochemistry and Molecular Biology,University of Chittagong,Chittagong-4331,Bangladesh
文献出处：
引用格式：
[1]Mamtaz Sultana;Nazmul Hasan;Mamunur R.Mahib;Mohammad M.Islam-.Systematic mutational analysis of epitope-grafted ED3 immunogenicity reveals a DENV3-DENV4 bi-serospecific ED3 mutant)[J].亚太热带医药杂志（英文版）,2022(02):中插1-中插4,63-70
A类：
DENV3,DENV4,serospecific,serospecificity,3ED3,4ED3,L304I,K305D,V309M,S310A,D383N,K384S,K387T,N389H,antisera,ED3s,4ED3epi1,DENVs
B类：
Systematic,mutational,analysis,grafted,immunogenicity,reveals,mutant,Objective,To,identify,residue,determinants,cross,reactivity,dengue,virus,envelope,protein,domain,which,contains,two,major,putative,epitopes,Methods,We,constructed,from,six,variants,where,transferred,onto,vice,versa,Swiss,albino,mice,aged,weeks,were,immunized,against,wildtype,IgG,antibody,responses,determined,using,Results,Mouse,immunization,generated,serotype,expected,Similarly,most,produced,template,little,recognition,taken,These,indicated,that,mere,grafting,was,not,sufficient,contrary,our,expectations,However,one,both,Conclusions,chimeric,produces,possessing,common,scaffold,therefore,provides,unique,tool,analyzing,believe,may,future,recombinant,multiple,single,pave,way,developing,tri,tetravalent
AB值：
0.343079
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