典型文献
An mRNA vaccine encoding Chikungunya virus E2-E1 protein elicits robust neutralizing antibody responses and CTL immune responses
文献摘要：
Arthropod-borne chikungunya virus(CHIKV)infection can cause a debilitating arthritic disease in human.However,there are no specific antiviral drugs and effective licensed vaccines against CHIKV available for clinical use.Here,we developed an mRNA-lipid nanoparticle(mRNA-LNP)vaccine expressing CHIKV E2-E1 antigen,and compared its immunogenicity with soluble recombinant protein sE2-El antigen expressed in S2 cells.For com-parison,we first showed that recombinant protein antigens mixed with aluminum adjuvant elicit strong antigen-specific humoral immune response and a moderate cellular immune response in C57BL/6 mice.Moreover,sE2-El vaccine stimulated 12-23 folds more neutralizing antibodies than sE1 vaccine and sE2 vaccine.Significantly,when E2-E1 gene was delivered by an mRNA-LNP vaccine,not only the better magnitude of neutralizing antibody responses was induced,but also greater cellular immune responses were generated,especially for CD8+T cell responses.Moreover,E2-E1-LNP induced CD8+T cells can perform cytotoxic effect in vivo.Considering its better immunogenicity and convenience of preparation,we suggest that more attention should be placed to develop CHIKV E2-E1-LNP mRNA vaccine.
文献关键词：
中图分类号：
作者姓名：
Ningning Ge;Jin Sun;Zhihua Liu;Jiayi Shu;Huimin Yan;Zhihua Kou;Yu Wei;Xia Jin
作者机构：
CAS Key Laboratory of Molecular Virology and Immunology,Institut Pasteur of Shanghai,Chinese Academy of Sciences,Shanghai 200031,China;University of Chinese Academy of Sciences,Beijing 100049,China;Shanghai Public Health Clinical Center,Fudan University,Shanghai 200540,China;Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China
文献出处：
引用格式：
[1]Ningning Ge;Jin Sun;Zhihua Liu;Jiayi Shu;Huimin Yan;Zhihua Kou;Yu Wei;Xia Jin-.An mRNA vaccine encoding Chikungunya virus E2-E1 protein elicits robust neutralizing antibody responses and CTL immune responses)[J].中国病毒学,2022(02):266-276
A类：
Arthropod,sE2,sE1
B类：
An,encoding,Chikungunya,virus,protein,elicits,robust,neutralizing,antibody,responses,CTL,immune,borne,chikungunya,CHIKV,infection,cause,debilitating,arthritic,disease,human,However,there,specific,antiviral,drugs,effective,licensed,vaccines,against,available,clinical,Here,developed,lipid,nanoparticle,LNP,expressing,compared,immunogenicity,soluble,recombinant,El,expressed,S2,cells,For,parison,first,showed,that,antigens,mixed,aluminum,adjuvant,strong,humoral,moderate,cellular,C57BL,mice,Moreover,stimulated,folds,more,antibodies,than,Significantly,when,was,delivered,by,not,only,better,magnitude,induced,but,also,greater,were,generated,especially,CD8+T,perform,cytotoxic,vivo,Considering,convenience,preparation,suggest,attention,should,placed
AB值：
0.521388
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