典型文献
Cryo-EM structures of human m6A writer complexes
文献摘要：
N6-methyladenosine(m6A)is the most abundant ribonucleotide modification among eukaryotic messenger RNAs.The m6A"writer"consists of the catalytic subunit m6A-METTL complex(MAC)and the regulatory subunit m6A-METTL-associated complex(MACOM),the latter being essential for enzymatic activity.Here,we report the cryo-electron microscopy(cryo-EM)structures of MACOM at a 3.0-A resolution,uncovering that WTAP and VIRMA form the core structure of MACOM and that ZC3H13 stretches the conformation by binding VIRMA.Furthermore,the 4.4-? resolution cryo-EM map of the MACOM-MAC complex,combined with crosslinking mass spectrometry and GST pull-down analysis,elucidates a plausible model of the m6A writer complex,in which MACOM binds to MAC mainly through WTAP and METTL3 interactions.In combination with in vitro RNA substrate binding and m6A methyltransferase activity assays,our results illustrate the molecular basis of how MACOM assembles and interacts with MAC to form an active m6A writer complex.
文献关键词：
中图分类号：
作者姓名：
Shichen Su;Shanshan Li;Ting Deng;Minsong Gao;Yue Yin;Baixing Wu;Chao Peng;Jianzhao Liu;Jinbiao Ma;Kaiming Zhang
作者机构：
State Key Laboratory of Genetic Engineering,Collaborative Innovation Center of Genetics and Development,Multiscale Research Institute of Complex Systems,Department of Biochemistry and Biophysics,School of Life Sciences,Fudan University,Shanghai,China;MOE Key Laboratory for Cellular Dynamics and Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui,China;MOE Key Laboratory of Macromolecular Synthesis and Functionalization,Department of Polymer Science and Engineering,Zhejiang University,Hangzhou,Zhejiang,China;National Facility for Protein Science in Shanghai,Zhangjiang Laboratory,Shanghai Advanced Research Institute,Chinese Academy of Science,Shanghai,China;Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation,Guangdong-Hong Kong Joint Laboratory for RNA Medicine,RNA Biomedical Institute,Medical Research Center,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou,Guangdong,China
文献出处：
引用格式：
[1]Shichen Su;Shanshan Li;Ting Deng;Minsong Gao;Yue Yin;Baixing Wu;Chao Peng;Jianzhao Liu;Jinbiao Ma;Kaiming Zhang-.Cryo-EM structures of human m6A writer complexes)[J].细胞研究（英文版）,2022(11):982-994
A类：
MACOM,VIRMA
B类：
Cryo,EM,structures,human,m6A,writer,complexes,N6,methyladenosine,most,abundant,ribonucleotide,modification,among,eukaryotic,messenger,RNAs,consists,catalytic,subunit,regulatory,associated,latter,being,essential,enzymatic,activity,Here,we,report,cryo,electron,microscopy,resolution,uncovering,that,WTAP,core,ZC3H13,stretches,conformation,by,binding,Furthermore,map,combined,crosslinking,mass,spectrometry,GST,pull,down,analysis,elucidates,plausible,model,which,binds,mainly,through,METTL3,interactions,In,combination,vitro,substrate,methyltransferase,assays,our,results,illustrate,molecular,basis,how,assembles,interacts,active
AB值：
0.530415
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
