典型文献
Cryo-EM structure of glycoprotein C from Crimean-Congo hemorrhagic fever virus
文献摘要：
Crimean-Congo hemorrhagic fever virus(CCHFV)is a causative agent of serious hemorrhagic diseases in humans with high mortality rates.CCHFV glycoprotein Gc plays critical roles in mediating virus-host membrane fusion and has been studied extensively as an immunogen.However,the molecular mechanisms involved in membrane fusion and Gc-specific antibody-antigen interactions remain unresolved largely because structural information of this glycoprotein is missing.We designed a trimeric protein including most of the ectodomain region of Gc from the prototype CCHFV strain,Ib Ar1 0200,which enabled the cryo-electron microscopy structure to be solved at a resolution of 2.8 ?.The structure confirms that CCHFV Gc is a class Ⅱ fusion protein.Unexpectedly,structural comparisons with other solved Gc trimers in the postfusion conformation revealed that CCHFV Gc adopted hybrid architectural features of the fusion loops from hantaviruses and domain Ⅲ from phenuiviruses,suggesting a complex evolutionary pathway among these bunyaviruses.Antigenic sites on CCHFV Gc that protective neutralizing antibodies target were mapped onto the CCHFV Gc structure,providing valuable information that improved our understanding of potential neutralization mechanisms of various antibodies.
文献关键词：
中图分类号：
作者姓名：
Na Li;Guibo Rao;Zhiqiang Li;Jiayi Yin;Tingting Chong;Kexing Tian;Yan Fu;Sheng Cao
作者机构：
CAS Key Laboratory of Special Pathogens,Center for Biosafety Mega-Science,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,430071,China;University of Chinese Academy of Sciences,Beijing,100049,China
文献出处：
引用格式：
[1]Na Li;Guibo Rao;Zhiqiang Li;Jiayi Yin;Tingting Chong;Kexing Tian;Yan Fu;Sheng Cao-.Cryo-EM structure of glycoprotein C from Crimean-Congo hemorrhagic fever virus)[J].中国病毒学,2022(01):127-137
A类：
ectodomain,postfusion,hantaviruses,phenuiviruses,bunyaviruses,Antigenic
B类：
Cryo,EM,structure,glycoprotein,from,Crimean,Congo,hemorrhagic,fever,CCHFV,causative,agent,serious,diseases,humans,high,mortality,rates,Gc,plays,critical,roles,mediating,host,membrane,has,been,studied,extensively,immunogen,However,molecular,mechanisms,involved,specific,antibody,antigen,interactions,remain,unresolved,largely,because,structural,information,this,missing,We,designed,trimeric,including,most,region,prototype,strain,Ib,Ar1,which,enabled,cryo,electron,microscopy,resolution,confirms,that,class,Unexpectedly,comparisons,other,trimers,conformation,revealed,adopted,hybrid,architectural,features,loops,suggesting,complex,evolutionary,pathway,among,these,sites,protective,neutralizing,antibodies,target,were,mapped,onto,providing,valuable,improved,our,understanding,potential,neutralization,various
AB值：
0.553757
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
