典型文献
The H3K9me2-binding protein AGDP3 limits DNA methylation and transcriptional gene silencing in ArabidopsisFA
文献摘要：
DNA methylation, a conserved epigenetic mark, is critical for tuning temporal and spatial gene expression. The Arabidopsis thaliana DNA gly-cosylase/lyase REPRESSOR OF SILENCING 1 (ROS1) initiates active DNA demethylation and is required to prevent DNA hypermethylation at thousands of genomic loci. However, how ROS1 is recruited to specific loci is not well understood. Here, we report the discovery of Arabidopsis AGENET Domain Containing Pro-tein 3 (AGDP3) as a cellular factor that is re-quired to prevent gene silencing and DNA hy-permethylation. AGDP3 binds to H3K9me2 marks in its target DNA via its AGD12 cassette. Analysis of the crystal structure of the AGD12 cassette of AGDP3 in complex with an H3K9me2 peptide revealed that dimethylated H3K9 and unmodified H3K4 are specifically anchored into two different surface pockets. A histidine residue located in the methyllysine binding aromatic cage provides AGDP3 with pH-dependent H3K9me2 binding capacity. Our results uncover a molecular mechanism for the regulation of DNA demethylation by the gene silencing mark H3K9me2.
文献关键词：
中图分类号：
作者姓名：
Xuelin Zhou;Mengwei Wei;Wenfeng Nie;Yue Xi;Li Peng;Qijie Zheng;Kai Tang;Viswanathan Satheesh;Yuhua Wang;Jinyan Luo;Xuan Du;Rui Liu;Zhenlin Yang;Honggui La;Yingli Zhong;Yu Yang;Jian-Kang Zhu;Jiamu Du;Mingguang Lei
作者机构：
Shanghai Center for Plant Stress Biology,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 201602,China;University of Chinese Academy of Sciences,Beijing 100049,China;Department of Horticulture,College of Horticulture and Plant Protection,Yangzhou University,Yangzhou 225009,China;Department of Horticulture&Landscape Architecture,Purdue University,West Lafayette,Idiana,47906,USA;Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes,Institute of Plant and Food Science,School of Life Sciences,Southern University of Science and Technology,Shenzhen 518055,China;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China
文献出处：
引用格式：
[1]Xuelin Zhou;Mengwei Wei;Wenfeng Nie;Yue Xi;Li Peng;Qijie Zheng;Kai Tang;Viswanathan Satheesh;Yuhua Wang;Jinyan Luo;Xuan Du;Rui Liu;Zhenlin Yang;Honggui La;Yingli Zhong;Yu Yang;Jian-Kang Zhu;Jiamu Du;Mingguang Lei-.The H3K9me2-binding protein AGDP3 limits DNA methylation and transcriptional gene silencing in ArabidopsisFA)[J].植物学报（英文版）,2022(12):2385-2395
A类：
AGDP3,ArabidopsisFA,cosylase,REPRESSOR,SILENCING,AGENET,permethylation,AGD12,dimethylated,methyllysine
B类：
H3K9me2,binding,protein,limits,transcriptional,silencing,conserved,epigenetic,critical,tuning,temporal,spatial,expression,thaliana,gly,lyase,OF,ROS1,initiates,active,demethylation,required,prevent,hypermethylation,thousands,genomic,loci,However,how,recruited,not,well,understood,Here,report,discovery,Domain,Containing,Pro,cellular,that,binds,marks,target,via,cassette,Analysis,crystal,structure,complex,peptide,revealed,unmodified,H3K4,are,specifically,anchored,into,two,different,surface,pockets,histidine,residue,located,aromatic,cage,provides,dependent,capacity,Our,results,uncover,molecular,mechanism,regulation,by
AB值：
0.466113
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