典型文献
Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex
文献摘要：
Nuclear pore complexes(NPCs)mediate bidirectional nucleocytoplasmic transport of substances in eukaryotic cells.However,the accurate molecular arrangement of NPCs remains enigmatic owing to their huge size and highly dynamic nature.Here we determined the structure of the asymmetric unit of the inner ring(IR monomer)at 3.73 A resolution by single-particle cryo-electron microscopy,and created an atomic model of the intact IR consisting of 192 molecules of 8 nucleoporins.In each IR monomer,the Z-shaped Nup188-Nup192 complex in the middle layer is sandwiched by two approximately parallel rhomboidal structures in the inner and outer layers,while Nup188,Nup192 and Nic96 link all subunits to constitute a relatively stable IR monomer.In contrast,the intact IR is assembled by loose and instable interactions between IR monomers.These structures,together with previously reported structural information of IR,reveal two distinct interaction modes between IR monomers and extensive flexible connections in IR assembly,providing a structural basis for the stability and malleability of IR.
文献关键词：
中图分类号：
作者姓名：
Zongqiang Li;Shuaijiabin Chen;Liang Zhao;Guoqiang Huang;Xiong Pi;Shan Sun;Peiyi Wang;Sen-Fang Sui
作者机构：
State Key Laboratory of Membrane Biology,Beijing Advanced Innovation Center for Structural Biology,Beijing Frontier Research Center for Biological Structure,School of Life Sciences,Tsinghua University,Beijing,China;Department of Biology,Southern University of Science and Technology,Shenzhen,Guangdong,China;Cryo-EM Center,Southern University of Science and Technology,Shenzhen,Guangdong,China
文献出处：
引用格式：
[1]Zongqiang Li;Shuaijiabin Chen;Liang Zhao;Guoqiang Huang;Xiong Pi;Shan Sun;Peiyi Wang;Sen-Fang Sui-.Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex)[J].细胞研究（英文版）,2022(05):437-450
A类：
nucleocytoplasmic,nucleoporins,Nup188,Nup192,rhomboidal,Nic96,malleability
B类：
Near,atomic,inner,ring,Saccharomyces,cerevisiae,nuclear,pore,Nuclear,complexes,NPCs,mediate,bidirectional,transport,substances,eukaryotic,cells,However,accurate,molecular,arrangement,remains,enigmatic,owing,their,huge,size,highly,dynamic,nature,Here,determined,asymmetric,resolution,by,single,particle,cryo,electron,microscopy,created,model,intact,consisting,molecules,In,each,shaped,middle,sandwiched,two,approximately,parallel,structures,outer,layers,while,link,subunits,constitute,relatively,contrast,assembled,loose,instable,interactions,between,monomers,These,together,previously,reported,structural,information,reveal,distinct,modes,extensive,flexible,connections,assembly,providing,basis,stability
AB值：
0.552786
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