典型文献
High-axial-resolution optical stimulation of neurons in vivo via two-photon optogenetics with speckle-free beaded-ring patterns
文献摘要：
Two-photon optogenetics has become an indispensable technology in neuroscience,due to its capability in precise and specific manipulation of neural activities.A scanless holographic approach is generally adopted to meet the requirement of stimulating neural ensembles simultaneously.However,the commonly used disk patterns fail in achieving single-neuron resolution,especially in axial dimension,and their inherent speckles decrease stimulation efficiency.Here,we propose a novel speckle-free,beaded-ring pattern for high-axial-resolution optical stimula-tion of neurons in vivo.Using a dye pool and a fluorescent thin film as samples,we verify that,compared to those with disk patterns,higher axial resolution and better localization ability can be achieved with beaded-ring patterns.Furthermore,we perform two-photon based all-optical physiology with neurons in mouse S1 cortex in vivo,and demonstrate that the axial resolution obtained by beaded-ring patterns can be improved by 24％when stimulating multiple neurons,compared to that of disk patterns.
文献关键词：
中图分类号：
作者姓名：
CHENG JIN;CHI LIU;LINGJIE KONG
作者机构：
State Key Laboratory of Precision Measurement Technology and Instruments,Department of Precision Instrument,Tsinghua University,Beijing 100084,China;IDG/McGovern Institute for Brain Research,Tsinghua University,Beijing 100084,China
文献出处：
引用格式：
[1]CHENG JIN;CHI LIU;LINGJIE KONG-.High-axial-resolution optical stimulation of neurons in vivo via two-photon optogenetics with speckle-free beaded-ring patterns)[J].光子学研究（英文）,2022(06):1367-1373
A类：
scanless
B类：
High,axial,resolution,optical,stimulation,neurons,vivo,via,two,photon,optogenetics,free,beaded,ring,patterns,Two,has,become,indispensable,technology,neuroscience,due,its,capability,precise,specific,manipulation,neural,activities,holographic,approach,generally,adopted,meet,requirement,stimulating,ensembles,simultaneously,However,commonly,used,disk,fail,achieving,single,especially,dimension,their,inherent,speckles,decrease,efficiency,Here,propose,novel,Using,dye,pool,fluorescent,thin,film,samples,verify,that,compared,those,higher,better,localization,achieved,Furthermore,perform,physiology,mouse,S1,cortex,demonstrate,obtained,by,improved,when,multiple
AB值：
0.514784
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