Quantitative phase imaging(QPI)is a label-free technique providing both morphology and quantitative biophysical information in biomedicine.However,applying such a powerful technique to in vivo pathological diagnosis remains challenging.Multi-core fiber bundles(MCFs)enable ultra-thin probes for in vivo imaging,but current MCF imaging techniques are limited to amplitude imaging modalities.We demonstrate a computational lensless microendoscope that uses an ultra-thin bare MCF to perform quantitative phase imaging with microscale lateral resolution and nanoscale axial sensitivity of the optical path length.The incident complex light field at the measurement side is precisely reconstructed from the far-field speckle pattern at the detection side,enabling digital refocusing in a multi-layer sample without any mechanical movement.The accuracy of the quantitative phase reconstruction is validated by imaging the phase target and hydrogel beads through the MCF.With the proposed imaging modality,three-dimensional imaging of human cancer cells is achieved through the ultra-thin fiber endoscope,promising widespread clinical applications.

Jiawei Sun;Jiachen Wu;Song Wu;Ruchi Goswami;Salvatore Girardo;Liangcai Cao;Jochen Guck;Nektarios Koukourakis;Juergen W.Czarske

Laboratory of Measurement and Sensor System Technique(MST),TU Dresden,Helmholtzstrasse 18,01069 Dresden,Germany;Competence Center for Biomedical Computational Laser Systems(BIOLAS),TU Dresden,Dresden,Germany;State Key Laboratory of Precision Measurement Technology and Instruments,Department of Precision Instruments,Tsinghua University,100084 Beijing,China;Institute for Integrative Nanosciences,IFW Dresden,HelmholtzstraBe 20,01069 Dresden,Germany;Max Planck Institute for the Science of Light&Max-Planck-Zentrum fur Physik und Medizin,91058 Erlangen,Germany;Cluster of Excellence Physics of Life,TU Dresden,Dresden,Germany;Institute of Applied Physics,TU Dresden,Dresden,Germany

光：科学与应用（英文版）

[1]Jiawei Sun;Jiachen Wu;Song Wu;Ruchi Goswami;Salvatore Girardo;Liangcai Cao;Jochen Guck;Nektarios Koukourakis;Juergen W.Czarske-.Quantitative phase imaging through an ultra-thin lensless fiber endoscope)[J].光：科学与应用（英文版）,2022(08):1843-1852

microendoscope

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