典型文献
Celastrol Induces Apoptosis and Autophagy via the AKT/mTOR Signaling Pathway in the Pituitary ACTH-secreting Adenoma Cells
文献摘要：
Objective:Pituitary adrenocorticotropic hormone(ACTH)-secreting adenoma is a relatively intractable endocrine adenoma that can cause a range of severe metabolic disorders and pathological changes involving multiple systems.Previous studies have shown that celastrol has antitumor effects on a variety of tumor cells via the AKT/mTOR signaling.However,whether celastrol has pronounced antitumor effects on pituitary ACTH-secreting adenoma is unclear.This study aimed to identify a new effective therapeutic drug for pituitary ACTH-secreting adenoma.Methods:Mouse pituitary ACTH-secreting adenoma cells(AtT20 cells)were used as an experimental model in vitro and to establish a xenograft tumor model in mice.Cells and animals were administered doses of celastrol at various levels.The effects of celastrol on cell viability,migration,apoptosis and autophagy were then examined.Finally,the potential involvement of AKT/mTOR signaling in celastrol's mechanism was assessed.Results:Celastrol inhibited the proliferation and migration of pituitary adenoma cells in a time-and concentration-dependent manner.It blocked AtT20 cells in the G0/G1 phase,and induced apoptosis and autophagy by downregulating the AKT/mTOR signaling pathway.Similar results were obtained in mice.Conclusion:Celastrol exerts potent antitumor effects on ACTH-secreting adenoma by downregulating the AKT/mTOR signaling in vitro and in vivo.
文献关键词：
中图分类号：
作者姓名：
Zhi CAI;Bin QIAN;Jing PANG;Zhou-bin TAN;Kai ZHAO;Ting LEI
作者机构：
Sino-German Neuro-Oncology Molecular Laboratory,Department of Neurosurgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Key Laboratory of Human Functional Genomics of Jiangsu Province,Biochemistry and Molecular Biology,Nanjing Medical University,Nanjing 211100,China;Department of Biochemistry and Molecular Cell Biology,Shanghai Jiao Tong University School of Medicine,Shanghai 200240,China
文献出处：
引用格式：
[1]Zhi CAI;Bin QIAN;Jing PANG;Zhou-bin TAN;Kai ZHAO;Ting LEI-.Celastrol Induces Apoptosis and Autophagy via the AKT/mTOR Signaling Pathway in the Pituitary ACTH-secreting Adenoma Cells)[J].当代医学科学(英文),2022(02):387-396
A类：
Adenoma
B类：
Celastrol,Induces,Apoptosis,Autophagy,AKT,mTOR,Signaling,Pathway,Pituitary,ACTH,secreting,Cells,Objective,adrenocorticotropic,hormone,adenoma,relatively,intractable,endocrine,that,can,cause,range,severe,metabolic,disorders,pathological,changes,involving,multiple,systems,Previous,studies,have,shown,celastrol,antitumor,effects,variety,cells,signaling,However,whether,pronounced,pituitary,unclear,This,study,aimed,identify,new,effective,therapeutic,drug,Methods,Mouse,AtT20,were,used,experimental,model,vitro,establish,xenograft,mice,animals,administered,doses,various,levels,viability,migration,apoptosis,autophagy,then,examined,Finally,potential,involvement,mechanism,was,assessed,Results,inhibited,proliferation,concentration,dependent,manner,It,blocked,G0,G1,phase,induced,by,downregulating,pathway,Similar,results,obtained,Conclusion,exerts,vivo
AB值：
0.520242
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