Colorectal cancer(CRC)is the third leading cancer globally.Metagenomics has been widely used to ana-lyze the association between the gut microbiota and CRC based on bacterial genus-or species-level com-parisons,providing evidence of dysbiosis in CRC development.However,this kind of analysis is unable to provide strain-level information for understanding the individual role of a species in CRC.Here,we used culturomics to isolate CRC mucosal samples and selected 158 Escherichia coli strains to reveal their dif-ferences in both genomics and functions by means of phylogenetic analysis and inflammatory induction based on cell and animal experiments.Through genomic comparison,these strains were divided into five phylogroups.The representative strains of each phylogroup significantly induced different levels of cyto-kine secretion by human leukemic monocyte(THP-1 cell)-based Transwell and animal experiments.Further bioinformatic analysis revealed different profiles of single-nucleotide polymorphisms,genes,and metabolic pathways in the different phylogroups,which can improve the current understanding of the phenotypic differences between these strains.The strain differences revealed in both genomics and functions indicate that the microbiota's function at the strain level should be investigated in order to understand the interacting mechanisms between hosts and gut bacteria.

Yuxiao Chang;Xiang Li;Lei Ding;Chao Yang;Zhiyuan Pan;Ni Han;Yujun Cui;Fachao Zhi;Ruifu Yang;Hong Gao;Yujing Bi

State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing 100071,China;Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China;Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China;Guangdong Provincial Key Laboratory of Gastroenterology,Institute of Gastroenterology of Guangdong Province,&Department of Gastroenterology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China

工程（英文）

[1]Yuxiao Chang;Xiang Li;Lei Ding;Chao Yang;Zhiyuan Pan;Ni Han;Yujun Cui;Fachao Zhi;Ruifu Yang;Hong Gao;Yujing Bi-.Genetic and Functional Differences of Escherichia coli Strains from Colorectal Cancer Mucosal Tissues)[J].工程（英文）,2022(09):210-219

Metagenomics,parisons,culturomics,phylogroups,phylogroup

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