Objective:The ataxia telangiectasia mutated(ATM)gene is a master regulator in cellular DNA damage response.The dysregulation of ATM expression is frequent in breast cancer,and is known to be involved in the carcinogenesis and prognosis of cancer.However,the underlying mechanism remains unclear.The bioinformatic analysis predicted a potential antisense transcript ATM-antisense(AS)from the opposite strand of the ATM gene.The purpose of this study was to identify ATM-AS and investigate the possible effect of ATM-AS on the ATM gene regulation.Methods:Single strand-specific RT-PCR was performed to verify the predicted antisense transcript ATM-AS within the ATM gene locus.qRT-PCR and Western blotting were used to detect the expression levels of ATM-AS and ATM in normal and breast cancer cell lines as well as in tissue samples.Luciferase reporter gene assays,biological mass spectrometry,ChIP-qPCR and RIP were used to explore the function of ATM-AS in regulating the ATM expression.Immunofluorescence and host-cell reactivation(HCR)assay were performed to evaluate the biological significance of ATM-AS in ATM-mediated DNA damage repair.Breast cancer tissue samples were used for evaluating the correlation of the ATM-AS level with the ATM expression as well as prognosis of the patients.Results:The ATM-AS significantly upregulated the ATM gene activity by recruiting KAT5 histone acetyltransferase to the gene promoter.The reduced ATM-AS level led to the abnormal downregulation of ATM expression,and impaired the ATM-mediated DNA damage repair in normal breast cells in vitro.The ATM-AS level was positively correlated with the ATM expression in the examined breast cancer tissue samples,and the patient prognosis.Conclusion:The present study demonstrated that ATM-AS,an antisense transcript located within the ATM gene body,is an essential positive regulator of ATM expression,and functions by mediating the binding of KAT5 to the ATM promoter.These findings uncover the novel mechanism underlying the dysregulation of the ATM gene in breast cancer,and enrich our understanding of how an antisense transcript regulates its host gene.

He CHENG;Er-shao ZHANG;Xiao SHI;Ping-ping CAO;Bei-jing PAN;Xin-xin SI;Yue LIU;Nan YANG;Ying CHU;Xu-chun WANG;Xiao HAN;Zhi-hong ZHANG;Yu-jie SUN

Key Laboratory of Human Functional Genomics of Jiangsu Province,Nanjing Medical University,Nanjing 210000,China;Department of Cell Biology,Nanjing Medical University,Nanjing 210000,China;Department of Pathology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210000,China;Key Laboratory of Cancer Biomarkers,Prevention and Treatment,Collaborative Innovation Center for Personalized Cancer Medicine,Nanjing Medical University,Nanjing 210000,China

当代医学科学(英文)

[1]He CHENG;Er-shao ZHANG;Xiao SHI;Ping-ping CAO;Bei-jing PAN;Xin-xin SI;Yue LIU;Nan YANG;Ying CHU;Xu-chun WANG;Xiao HAN;Zhi-hong ZHANG;Yu-jie SUN-.A Novel ATM Antisense Transcript ATM-AS Positively Regulates ATM Expression in Normal and Breast Cancer Cells)[J].当代医学科学(英文),2022(04):681-691

Antisense,Regulates

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