Objective:Gastric cancer(GC)is a deadly cancer and a challenging public health problem globally.This study aimed to analyze potential genes associated with pathogenesis and prognosis of gastric cancer.Methods:This work selected the overlapping differentially expressed genes(DEGs)in GC from four datasets,the GSE29272,GSE29998,GSE54129 and GSE118916 Gene Expression Omnibus databases.These DEGs were used to carry out comprehensive bioinformatic analysis to analyze the related functions and pathways enriched,the relative expression levels and immune infiltrates,the prognostic characteristics and the interaction network.Results:In total,55 DEGs increased while 98 decreased in their expression levels.For those DEGs with increased expression,they were mostly concentrated on"focal adhesion"and"ECM-receptor interaction",whereas DEGs with decreased expression were mostly associated with"gastric acid secretion"and"drug metabolism cytochrome P450".MCODE and ClueGO results were then integrated to screen 10 hub genes,which were FN1,COL1A1,COL3A1,BGN,TIMP1,COL1A2,LUM,VCAN,COL5A2 and SPP1.Survival analysis revealed that higher expression of the ten hub genes significantly predicted lower overall survival of GC patients.TIMP1 was most significantly related to neutrophils,CD8+T cells,as well as dendritic cells,while LUM was most significantly related to macrophages.Conclusion:Immunohistochemistry results and functional testing showed that the expression of COL5A2 was elevated in GC and that it might be a key gene in GC tumorigenesis.

Dan-wen WANG;Fei SU;Li-jie YANG;Li-wen SHI;Tie-cheng YANG;Hua-qiao WANG;Xuan-fei LI;Mao-hui FENG

Department of Gastrointestinal Surgery,Zhongnan Hospital of Wuhan University,Wuhan 443071,China;Clinical Medical Research Center of Peritoneal Cancer of Wuhan,Wuhan 443071,China;Clinical Cancer Study Center of Hubei Provence,Wuhan 443071,China;Hubei Key Laboratory of Tumor Biological Behaviors,Wuhan 443071,China;Department of Oncology,the First Hospital of Lanzhou University,Lanzhou 730000,China

当代医学科学(英文)

[1]Dan-wen WANG;Fei SU;Li-jie YANG;Li-wen SHI;Tie-cheng YANG;Hua-qiao WANG;Xuan-fei LI;Mao-hui FENG-.Bioinformatics Analysis and Identification of Potential Genes Associated with Pathogenesis and Prognosis of Gastric Cancer)[J].当代医学科学(英文),2022(02):357-372

GSE29272,GSE29998

Bioinformatics,Analysis,Identification,Potential,Genes,Associated,Pathogenesis,Prognosis,Gastric,Cancer,Objective,cancer,deadly,challenging,public,health,problem,globally,This,study,aimed,analyze,potential,associated,pathogenesis,prognosis,gastric,Methods,selected,overlapping,differentially,expressed,DEGs,from,four,datasets,GSE54129,GSE118916,Expression,Omnibus,databases,These,were,used,carry,out,comprehensive,bioinformatic,analysis,related,functions,pathways,enriched,relative,expression,levels,immune,infiltrates,prognostic,characteristics,interaction,network,Results,In,total,increased,while,decreased,their,For,those,they,mostly,concentrated,focal,adhesion,ECM,receptor,whereas,acid,secretion,drug,metabolism,cytochrome,P450,MCODE,ClueGO,results,then,integrated,screen,hub,which,FN1,COL1A1,COL3A1,BGN,TIMP1,COL1A2,LUM,VCAN,COL5A2,SPP1,Survival,revealed,that,higher,significantly,predicted,lower,overall,survival,patients,was,neutrophils,CD8+T,cells,well,dendritic,macrophages,Conclusion,Immunohistochemistry,functional,testing,showed,elevated,might,be,key,tumorigenesis

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