Increasing evidence has indicated that there is a correlation between Fusobacterium nucleatum(F.nucleatum)abundance and poor prognosis of colorectal cancer(CRC).Furthermore,tumor metastasis plays a decisive role in the prognosis of CRC patients.Therefore,it was hypothesized that the abundance of F nucleatum in CRC tissues affects the tumor metastasis.Methods:In the present study,F nucleatum DNA obtained from 141 resected CRC samples was quantified by qPCR to determine whether there were differences in F nucleatum abundance between groups with and without CRC metastasis.Results:The results revealed that F nucleatum was more abundant in CRC patients with metastasis,and CRC tissues enriched with F.nucleatum had a higher risk of lymph node metastasis and distant metastasis.The receiver operating characteristic curve indicated that F nucleatum in CRC tissues could be used as an indicator for CRC metastasis,to some extent.Furthermore,the in vitro experiments(electron microscopy,and migration and invasion trials)revealed that F nucleatum was a highly invasive bacterial strain,and could significantly enhance the invasion and migration capacity of SW480 and SW620 cells.In addition,a meta-analysis comprehensively indicated a slight correlation between F nucleatum abundance and advanced CRC stage(RR=1.17,95％CI:1.00-1.37,P=0.04,random effect).Conclusion:There is a correlation between F nucleatum abundance and CRC metastasis,and F nucleatum may serve as a metastasis biomarker for CRC patients.

Wen-dan CHEN;Xin ZHANG;Ya-ping ZHANG;Cong-bo YUE;Yan-lei WANG;Hong-wei PAN;Yan-li ZHANG;Hui LIU;Yi ZHANG

Department of Clinical Laboratory,Qilu Hospital of Shandong University,Jinan 250012,China;Department of General Surgery,Qilu Hospital of Shandong University,Jinan 250012,China;Department of Clinical Laboratory,Shandong Provincial Third Hospital,Jinan 250031,China

当代医学科学(英文)

[1]Wen-dan CHEN;Xin ZHANG;Ya-ping ZHANG;Cong-bo YUE;Yan-lei WANG;Hong-wei PAN;Yan-li ZHANG;Hui LIU;Yi ZHANG-.Fusobacterium Nucleatum Is a Risk Factor for Metastatic Colorectal Cancer)[J].当代医学科学(英文),2022(03):538-547

Nucleatum

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