Due to the worldwide epidemic of allergic disease and a cure nowhere in sight,there is a crucial need to explore its pathophysiological mechanisms.As allergic disease has been associated with gut dysbiosis,we searched for a possible mechanism from the perspective of the molecular interface between host and microbiota with concurrent metabolomics and microbiome composition analysis.Sprague-Dawley rats were injected with Artemisia pollen extract to stimulate a hyper reaction to pollen.This hyper reaction decreased the circulation of valine,isoleucine,aspartate,glutamate,glutamine,indole-propionate(IPA),and myo-inositol,and reduced short-chain fatty acids(SCFAs)in feces.Several beneficial genera belonging to Ruminococcaceae,Lachnospiraceae,and Clostridiales declined in the model group,whereas Helicobacter and Akkermansia were only expressed in the model group.Furthermore,the expression of intestinal claudin-3 and liver fatty acid binding protein was downregulated in the model group and associated with metabolic changes and bacteria.Our results suggest that alterations in amino acids as well as their derivatives(especially valine,and IPA which is the reductive product of tryptophan),SCFAs,and the gut microbiome(specifically Akkermansia and Helicobacter)may disrupt the intestinal barrier function by inhibiting the expression of claudin proteins and affecting the mucus layer,which further results in hay fever.

Pei Han;Li-Sha Li;Zi-Xi Wang;Lin Xi;Hang Yu;Lin Cong;Zheng-Wei Zhang;Jie Fu;Ran Peng;Li-Bin Pan;Shu-Rong Ma;Xue-Yan Wang;Hong-Tian Wang;Xiang-Dong Wang;Yan Wang;Jin-Lyu Sun;Jian-Dong Jiang

State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China;Department of Allergy,National Clinical Research Center for Dematologic and Immunologic Diseases,Beijing Key Laboratory of Precision Medicine for Diagnosis and Treatment on Allergic Diseases,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100730,China;Department of Allergy&Department of Otorhinolaryngology Head and Neck Surgery&Key Laboratory of Otolaryngology Head and Neck Surgery,Ministry of Education,Beijing Tongren Hospital,Capital Medical University,Beijing 100005,China;Department of Allergy,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China;Beijing Key Laboratory of Nasal Diseases,Beijing Institute of Otolaryngology,Beijing Tongren Hospital,Capital Medical University,Beijing 100005,China

工程（英文）

[1]Pei Han;Li-Sha Li;Zi-Xi Wang;Lin Xi;Hang Yu;Lin Cong;Zheng-Wei Zhang;Jie Fu;Ran Peng;Li-Bin Pan;Shu-Rong Ma;Xue-Yan Wang;Hong-Tian Wang;Xiang-Dong Wang;Yan Wang;Jin-Lyu Sun;Jian-Dong Jiang-.Multi-Omics Analysis Provides Insight into the Possible Molecular Mechanism of Hay Fever Based on Gut Microbiota)[J].工程（英文）,2022(08):115-125

Multi,Omics,Analysis,Provides,Insight,into,Possible,Molecular,Mechanism,Hay,Fever,Based,Gut,Microbiota,Due,worldwide,epidemic,allergic,disease,cure,nowhere,there,crucial,need,explore,its,pathophysiological,mechanisms,has,been,associated,gut,dysbiosis,searched,possible,from,perspective,molecular,interface,between,host,microbiota,concurrent,metabolomics,microbiome,composition,analysis,Sprague,Dawley,rats,were,injected,Artemisia,pollen,extract,stimulate,hyper,reaction,This,decreased,circulation,valine,isoleucine,aspartate,glutamate,glutamine,indole,propionate,IPA,myo,inositol,reduced,short,chain,fatty,acids,SCFAs,feces,Several,beneficial,genera,belonging,Ruminococcaceae,Lachnospiraceae,Clostridiales,declined,model,group,whereas,Helicobacter,Akkermansia,only,expressed,Furthermore,expression,intestinal,claudin,liver,binding,was,downregulated,metabolic,changes,bacteria,Our,results,suggest,that,alterations,amino,well,their,derivatives,especially,which,reductive,product,tryptophan,specifically,may,disrupt,barrier,function,by,inhibiting,proteins,affecting,mucus,layer,further,hay,fever

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