Alzheimer's disease(AD)is the most common age-related neuro-degenerative disorder.Emerging evidence suggests that synaptic dysfunction is associated with the onset and progression of AD.Interestingly,Ginkgo biloba extract(EGb)is one of the most frequently investigated herbal medicines for enhancing cognition and alleviating neurodegenerative dementia.This study aimed to investigate the effect and the mechanism of EGb on AD-like synaptic disorders.Methods:Scopolamine(SCO)-induced rats were used to mimic AD-like memory deficits.Morris water maze test and fear conditioning test were conducted to evaluate the memory status of rats in response to different treatments.Then,the synapse alterations were assessed by Golgi staining,and Western blotting was conducted to assess the protein expression of PSD95,GluN2B,synapsin-1,and synaptophysin.Reverse transcription quantitative polymerase chain reaction was applied to detect the mRNA expression of PSD95 and the levels of miR-1-3p/miR-206-3p.Results:EGb supplement alleviated the learning and memory deficits induced by SCO in behavioral experiments.Moreover,EGb treatment attenuated synaptic damage elicited by SCO,manifested as increased dendritic spine density and the proportion of mushroom-type spines in hippocampal neurons.Further investigation indicated that EGb rescued the expression of synaptic-related proteins,especially PSD95,and decreased the levels of miR-1-3p/miR-206-3p in the rat hippocampus.Conclusion:The application of EGb effectively treats SCO-induced memory impairments probably by suppressing miR-1-3p/miR-206-3p and elevating the expression ofPSD95.

Gui-jiao ZHANG;Dong ZHENG;Huan YU;Xiao-ping LUO;Wei WU

Department of Pediatrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Department of Orthopedics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

当代医学科学(英文)

[1]Gui-jiao ZHANG;Dong ZHENG;Huan YU;Xiao-ping LUO;Wei WU-.Ginkgo Biloba Extract Ameliorates Scopolamine-induced Memory Deficits via Rescuing Synaptic Damage)[J].当代医学科学(英文),2022(03):474-482

Biloba,Scopolamine,Deficits,Rescuing,EGb,ofPSD95

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