典型文献
Dieckol isolated from Eisenia bicyclis extract suppresses RANKL-induced osteoclastogenesis in murine RAW 264.7 cells
文献摘要：
Objective: To demonstrate the effect of dieckol from Eisenia bicyclis on osteoclastogenesis using RAW 264.7 cells.Methods: Murine macrophage RAW 264.7 cells were subjected to dieckol treatment, followed by treatment with receptor activator of nuclear factor kappa-B ligand (RANKL) to induce osteoclastogenesis. Tartrate-resistant acid phosphatase (TRAP) activity was examined using a TRAP activity kit. Western blotting analysis was conducted to examine the level of osteoclast-related factors, including TRAP and calcitonin receptor (CTR), transcriptional factors, including c-Fos, c-Jun, and nuclear factor of activated T cells cytoplasmic 1 (NFATc1), nuclear factor kappa-B (NF-κB), extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK). Immunofluorescence staining was conducted to examine the expression of c-Fos, c-Jun, and NFATc1. Results: Among the four phlorotannin compounds present in Eisenia bicyclis, dieckol significantly hindered osteoclast differentiation and expression of RANKL-induced TRAP and CTR. In addition, dieckol downregulated the expression levels of c-Fos, c-Jun, NFATc1, ERK, and JNK, and suppressed NF-κB signaling.Conclusions: Dieckol can suppress RANKL-induced osteoclastogenesis. Therefore, it has therapeutic potential in treating osteoclastogenesis-associated diseases.
文献关键词：
中图分类号：
作者姓名：
Su-Hyeon Cho;Tae-Hyung Kwon;Hoibin Jeong;Jin Sook Kim;Song-Rae Kim;Myeong Seon Jeong;SeonJu Park;Miri Choi;Jung-Hee Woo;Juhee Ahn;Kil-Nam Kim
作者机构：
Chuncheon Center,Korea Basic Science Institute,Chuncheon 24341,Republic of Korea;Department of Medical Biomaterials Engineering,College of Biomedical Sciences,Kangwon National University,Chuncheon 24341,Republic of Korea;Department of Research and Development,Chuncheon Bio-industry Foundation,Chuncheon,Republic of Korea;Marine Industry Research Institute for East se rim,Uljin-gun,Gyeongsangbuk-do,Republic of Korea;Department of Bio-analysis Science,University of Science&Technology,Daejeon,34113,Republic of Korea
文献出处：
引用格式：
[1]Su-Hyeon Cho;Tae-Hyung Kwon;Hoibin Jeong;Jin Sook Kim;Song-Rae Kim;Myeong Seon Jeong;SeonJu Park;Miri Choi;Jung-Hee Woo;Juhee Ahn;Kil-Nam Kim-.Dieckol isolated from Eisenia bicyclis extract suppresses RANKL-induced osteoclastogenesis in murine RAW 264.7 cells)[J].亚太热带生物医学杂志（英文版）,2022(06):262-269
A类：
Dieckol,bicyclis,dieckol,phlorotannin
B类：
isolated,from,Eisenia,extract,suppresses,RANKL,induced,osteoclastogenesis,murine,RAW,cells,Objective,To,demonstrate,effect,using,Methods,Murine,macrophage,were,subjected,treatment,followed,by,receptor,activator,nuclear,kappa,ligand,Tartrate,resistant,acid,phosphatase,TRAP,activity,was,examined,kit,blotting,analysis,conducted,related,factors,including,calcitonin,CTR,transcriptional,Fos,Jun,activated,cytoplasmic,NFATc1,extracellular,kinase,ERK,terminal,JNK,Immunofluorescence,staining,expression,Results,Among,four,compounds,present,significantly,hindered,differentiation,In,addition,downregulated,levels,suppressed,signaling,Conclusions,Therefore,has,therapeutic,potential,treating,associated,diseases
AB值：
0.462176
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