OBJECTIVE:To study the effects and mechanism of Shenqihuatan formula(参七化痰方,SQHT)of the transforming growth factor-beta(TGF-β)-stimulated cell processes in airway remodeling.METHODS:The current study examined cell viability using a Cell Counting Kit-8 assay.Furthermore,a Transwell assay was conducted to detect the ability of cell migration,and apoptosis was detected via flowcytometry.Western Blot and quantitative real-time polymerase chain reaction(qRT-PCR)were used to determine the expression levels of apoptosis or inflammation-related factors,such as TGF-β,lnterleukin-1 β(IL-1β),B cell lymphoma 2(Bcl-2),Bcl-2-Associated X(Bax),Ras homolog gene family,member A(RhoA),recombinant rho associated coiled coil containing protein kinase 1/2(ROCK1/2),extracellular regulated protein kinases 1/2(ERK1/2),Snail,and Slug.Finally,the expression levels of matrix metalloproteinase-9(MMP-9)and Tissue inhibitor of metalloproteinase(TIMP-1)were admeasured by enzyme-linked immuno sorbent assay.RESULTS:The results demonstrated that SQHT inhibited the viability and migration,as well as the the F-actin formation and cytoskeletal reorganization of airway smooth muscle cells(ASMCs)stimulated by TGF-β.By monitoring the changes of critical regulators in the presence of the formula,it was observed that the expression levels of TGF-β,IL-1β,Bcl-2,RhoA,ROCK1/2,ERK1/2,Snail,and Slug were markedly suppressed,whereas Bax expression exhibited the opposite effect.Compared with a well-characterized RhoA pathway inhibitor,Fasudil,SQHT generated equivalent or even higher inhibitory effects on these processes in ASMCs.CONCLUSIONS:Collectively,these suggested that SQHT can reduce airway inflammation by inhibiting TGF-β-stimulated signaling pathways in ASMCs.These findings may provide a novel remedy for treating ASMC inflammation,which causes thickening and obstruction of the airway in chronic obstructive pulmonary disease.

CHEN Jingjing;WANG Yuanyuan;ZHANG Nianzhi;XUE Xiaoming

Shanxi Provincial Traditional Chinese Medicine Hospital,Taiyuan 030012,China;Anhui University of Traditional Chinese Medicine,Hefei 230038,China;Department of Respiratory,the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Hefei 230031,China

中医杂志（英文版）

[1]CHEN Jingjing;WANG Yuanyuan;ZHANG Nianzhi;XUE Xiaoming-.Shenqihuatan formula(参七化痰方)reduces inflammation by inhibiting transforming growth factor-beta-stimulated signaling pathway in airway smooth muscle cells)[J].中医杂志（英文版）,2022(04):520-529

Shenqihuatan,SQHT,flowcytometry,lnterleukin,admeasured

formula,化痰,reduces,inflammation,by,inhibiting,transforming,growth,beta,stimulated,signaling,airway,smooth,muscle,cells,OBJECTIVE,To,study,effects,mechanism,TGF,processes,remodeling,METHODS,current,examined,viability,using,Cell,Counting,Kit,assay,Furthermore,Transwell,was,conducted,migration,apoptosis,detected,Blot,quantitative,real,polymerase,chain,reaction,qRT,were,used,determine,expression,levels,related,factors,such,lymphoma,Bcl,Associated,Bax,Ras,homolog,family,member,RhoA,recombinant,rho,associated,coiled,containing,ROCK1,extracellular,regulated,kinases,ERK1,Snail,Slug,Finally,matrix,metalloproteinase,MMP,Tissue,TIMP,enzyme,linked,immuno,sorbent,RESULTS,results,demonstrated,that,inhibited,actin,formation,cytoskeletal,reorganization,ASMCs,By,monitoring,changes,critical,regulators,presence,observed,markedly,suppressed,whereas,exhibited,opposite,Compared,characterized,Fasudil,generated,equivalent,even,higher,inhibitory,these,CONCLUSIONS,Collectively,suggested,can,pathways,These,findings,may,provide,novel,remedy,treating,which,causes,thickening,obstruction,chronic,obstructive,pulmonary,disease

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