典型文献
Development of two multiplex real-time PCR assays for simultaneous detection and differentiation of monkeypox virus Ⅱa,Ⅱb,and Ⅰ clades and the B.1 lineage
文献摘要：
An ongoing multi-country outbreak of monkeypox was reported in May 2022 with several deaths,affecting 107 countries of all six World Health Organization(WHO)regions.The WHO has declared the current monkeypox outbreak to be a Public Health Emergency of International Concern.It is,thus,necessary to rapidly and accu-rately detect and distinguish different monkeypox virus(MPXV)clades.We designed primers and probes based on the alignment of 138 complete genomes of poxviruses.In Panel 1,we mixed one pair of primers and three probes to detect and differentiate the MPXV Western Africa(Ⅱa,Ⅱb clade)and Congo Basin(Ⅰ clade)and other orthopoxviruses.In Panel 2,we mixed one pair of primers and two probes to detect the 2022 MPXV(B.1 lin-eage and its descendant lineages).In addition,we tested the specificity and sensitivity of the assay using real-time PCR.In Panel 1,the assay reproducibly identified various concentrations of two plasmids of the monkey-pox virus,whereas other orthopoxviruses did not cross-react.In Panel 2,the probe annealed well to MPXV B.1 and showed the expected linearity.These two multiple real-time assays are inclusive and highly specific for identifying different clades of MPXV.
文献关键词：
中图分类号：
作者姓名：
Shuting Huo;Yuda Chen;Roujian Lu;Zhongxian Zhang;Gaoqian Zhang;Li Zhao;Yao Deng;Changcheng Wu;Wenjie Tan
作者机构：
NHC Key Laboratory of Biosafety,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China;School of Public Health,Baotou Medical College,Baotou 014030,China;Department of Microbiology,Basic Medical College,Inner Mongolia Medical University,Hohhot010010,China
文献出处：
引用格式：
[1]Shuting Huo;Yuda Chen;Roujian Lu;Zhongxian Zhang;Gaoqian Zhang;Li Zhao;Yao Deng;Changcheng Wu;Wenjie Tan-.Development of two multiplex real-time PCR assays for simultaneous detection and differentiation of monkeypox virus Ⅱa,Ⅱb,and Ⅰ clades and the B.1 lineage)[J].生物安全与健康 （英文）,2022(06):392-398
A类：
poxviruses,orthopoxviruses,eage,descendant,reproducibly
B类：
Development,two,multiplex,real,assays,simultaneous,detection,differentiation,monkeypox,clades,An,ongoing,country,outbreak,was,reported,May,several,deaths,affecting,countries,all,six,World,Health,Organization,WHO,regions,has,declared,current,Public,Emergency,International,Concern,It,thus,necessary,rapidly,accu,rately,distinguish,MPXV,designed,primers,probes,alignment,complete,genomes,Panel,mixed,one,pair,three,differentiate,Africa,Congo,Basin,other,its,lineages,addition,tested,specificity,sensitivity,using,identified,various,concentrations,plasmids,whereas,did,not,cross,react,annealed,well,showed,expected,linearity,These,inclusive,highly,identifying
AB值：
0.469161
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