OBJECTIVE:To evaluate the compatibility of Tianma(Rhizoma Gastrodiae,TM),Yanlingcao(Trillium tschonoskii Maxim,YLC)and Bingpian(Bomneolum Syntheticum,BP),and their efficacy in the treatment of cerebral ischemic stroke.METHODS:Network pharmacology was used to determine the compatibility of TM,YLC,and BP,and their potential mechanism.The middle cerebral artery occlusion(MCAO)rat model was used to evaluate the curative effect of the six combinations of TM,YLC,and BP(TZB1-TZB6)on cerebral ischemia,by using the weight matching method to form.The potential component changes of TM and YLC in the blood and brains of rats were analyzed using ultra performance liquid chromatography-mass spectrometry.Finally,molecular docking linked the results of animal experiments and network pharmacology,determining the potential component contributors of TM and YLC to treating ischemic stroke.RESULTS:TZB reduced the cerebral infarct volume and protected the nerve cells in MCAO rats.The components of TM and YLC were also identified in the blood and brain homogenate,and BP can facilitate the entry of the components of TM and YLC into the blood and brain.Diosgenin,pennogenin,and gastrodin induced effective binding activities with adenosine receptor al.CONCLUSION:We investigate an approach that improves the means of folk prescription combined with multi technology that maybe promote the transformation of Chinese medicinal prescription into component-based Chinese medicine.

LI Zhiyong;ZHU Na;LI Jianliang;FENG Liang;JIANG Yanyan;LI Caifeng;LIN Ling;HUANG Xiulan

Postdoctoral mobile station of environmental biology,Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming,Yunnan 650500,China;Center for Analytical Chemistry of Chinese Materia Medica,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Pharmacological Department,School of Pharmacy,Minzu University of China,Beijing,100081,China;Aerosol preparation research center,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing,Jiangsu 211198,China;Department of Chinese Materia Medica Chemistry,School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing,102488,China;Academician work station,Jiangxi University of Traditional Chinese Medicine,Nanchang,Jiangxi,330004,China

中医杂志（英文版）

[1]LI Zhiyong;ZHU Na;LI Jianliang;FENG Liang;JIANG Yanyan;LI Caifeng;LIN Ling;HUANG Xiulan-.Effcacy-oriented compatibility for Tianma(Rhizoma Gastrodiae),Yanlingcao(Trillium tschonoskii Maxim)and Bingpian(Borneolum Syntheticum)on improving cerebral ischemia stroke by network pharmacology and serum pharmacological methods)[J].中医杂志（英文版）,2022(03):408-416

Effcacy,Gastrodiae,Yanlingcao,Bingpian,Borneolum,Syntheticum,Bomneolum,TZB1,TZB6,TZB,Diosgenin,pennogenin

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