典型文献
Hyperpolarization-activated cyclic nucleotide-gated 2 contributes to electroacupuncture analgesia on lumbar disc herniation-induced radicular pain through activation of microglia in spinal dorsal horn
文献摘要：
OBJECTIVE:To explore the mechanisms of dorsal root ganglia and spinal microglia cascade cross in electroacupuncture(EA)analgesia in the treatment of lumbar disc herniation.METHODS:A rat model of lumbar disc herniation(LDH)was established,EA was administered at Huantiao(GB30)acupoint 30 min once a day,for 3 d.Before and after modeling,and after EA,mechanical allodynia thresholds were detected.Hyperpolarization-activated cyclic nucleotide-gated 2(HCN2)in dorsal root ganglia was detected by quantitative polymerase chain reaction(qPCR)and Western blot.C-X3-C motif chemokine ligand 1(CX3CL1)and activity of microglia in spinal cord was observed separately via qPCR and immunofluorescence staining.RESULTS:The mechanical allodynia threshold of the right planta of model rats was significantly reduced(P＜0.01),EA increased the mechanical pain threshold of rats(P＜0.01),and decreased HCN2 mRNA,and protein expression,reduced the expression of CX3CL1 and the activation of microglia.ZD7288(a blocker of HCN channel)reduced the analgesic effect of EA from 1.83±0.84 to 0.74±0.20(P＜0.05),and the expression of CX3CL1 in the spinal cord decreased from 0.52±0.11 to 0.15±0.05(P＜0.01).CONCLUSION:EA analgesia on the radicular pain of LDH is definite.EA reduced the expression of HCN2 channel in the dorsal root ganglion,thereby decreasing the noxious stimulation entered to microglia in spinal dorsal horn.Our work supports EA is an effective treatment for radicular pain of LDH.
文献关键词：
中图分类号：
作者姓名：
QIN Qingguang;CHEN Zujiang;FAN Weimin;LI Junhua;LIAO Liqing;LI Yikai
作者机构：
School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China
文献出处：
引用格式：
[1]QIN Qingguang;CHEN Zujiang;FAN Weimin;LI Junhua;LIAO Liqing;LI Yikai-.Hyperpolarization-activated cyclic nucleotide-gated 2 contributes to electroacupuncture analgesia on lumbar disc herniation-induced radicular pain through activation of microglia in spinal dorsal horn)[J].中医杂志（英文版）,2022(03):372-378
A类：
Hyperpolarization,radicular,Huantiao
B类：
activated,cyclic,nucleotide,gated,contributes,electroacupuncture,analgesia,lumbar,disc,herniation,induced,pain,through,activation,microglia,spinal,dorsal,horn,OBJECTIVE,To,explore,mechanisms,root,ganglia,cascade,cross,EA,treatment,METHODS,LDH,was,established,administered,GB30,acupoint,once,day,Before,after,modeling,mechanical,allodynia,thresholds,were,detected,HCN2,quantitative,polymerase,chain,reaction,qPCR,blot,motif,chemokine,ligand,CX3CL1,activity,cord,observed,separately,via,immunofluorescence,staining,RESULTS,right,planta,rats,significantly,reduced,increased,decreased,protein,expression,ZD7288,blocker,channel,analgesic,from,CONCLUSION,definite,ganglion,thereby,decreasing,noxious,stimulation,entered,Our,work,supports,effective
AB值：
0.450562
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