Objective:To observe the effect of electroacupuncture(EA)combined with oriented conductive bio-protein hydrogel(OCBH)on the recovery of nerve function in rats with complete spinal cord injury(SCI)and to explore its effect and mechanism on the formation and changes of glial scars.Methods:A total of 72 female Sprague-Dawley rats were randomly divided into groups according to the treatment received.A rat model of complete SCI was constructed using a spinal cord transection.Behavioral assessments,hematoxylin-eosin(H&E)staining,immunofluorescence staining,and Western blotting were performed at a fixed period after the operation.Results:The material group and the material+EA group obtained better results in the behavioral as-sessments(all P＜.05)and the H&E staining.In the immunofluorescence staining and Western blotting,the GFAP protein was expressed more and denser in the material group and the material+EA group than in the model group,and the density of the GFAP expression in the material+EA group was lower at week 12 than in the material group(all P＜.05).The expression of complement C3 in the model,material,and material+EA groups decreased in turn.Some inflammatory factors and the NF-kB signaling pathway showed similar results in the Western blotting(all P＜.05).The expression of the GDNF protein in the material+EA group was significantly higher than that in the model group and the material group(both P＜.01).Conclusion:EA combined with OCBH can promote the recovery of motor functions after SCI by facili-tating the formation of glial scars in the early stage,preventing the further spread of an inflammatory response that would affect the activation of A1/A2 astrocytes and change the morphology of glial scars at the spinal cord-material interface in its late stage.

Kaitan Yang;Yushan Gao;Yongdong Yang;Chuanhong Li;Zheng Cao;Xiumei Wang;Zaibo Li;Xing Yu

Ward Three,Department of Orthopedics,Beijing University of Chinese Medicine Dongzhimen Hospital,100010,Beijing,China;School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,100029,Beijing,China;School of Materials Science and Engineering,Tsinghua University,100084,Beijing,China;Department of Pathology,The OHIO State University Wexner Medical Center,Columbus,OH,43210,USA

中医科学杂志（英文）

[1]Kaitan Yang;Yushan Gao;Yongdong Yang;Chuanhong Li;Zheng Cao;Xiumei Wang;Zaibo Li;Xing Yu-.Effects of electroacupuncture combined with hydrogel on the formation and changes in the glial scar in rats with spinal cord injury)[J].中医科学杂志（英文）,2022(02):197-205

OCBH,material+EA,sessments,tating

Effects,electroacupuncture,combined,hydrogel,formation,changes,glial,rats,spinal,injury,Objective,To,observe,effect,oriented,conductive,bio,protein,recovery,nerve,complete,SCI,explore,its,mechanism,scars,Methods,total,female,Sprague,Dawley,were,randomly,divided,into,groups,according,treatment,received,model,was,constructed,using,transection,Behavioral,assessments,hematoxylin,eosin,staining,immunofluorescence,blotting,performed,fixed,period,after,operation,Results,obtained,better,results,behavioral,all,In,GFAP,expressed,more,denser,than,density,expression,lower,week,complement,C3,decreased,turn,Some,inflammatory,factors,kB,signaling,pathway,showed,similar,GDNF,significantly,higher,that,both,Conclusion,promote,motor,functions,by,facili,early,stage,preventing,further,spread,response,would,affect,activation,A1,A2,astrocytes,morphology,interface,late

0.436281