典型文献
Interleukin-4 promotes microglial polarization toward a neuroprotective phenotype after retinal ischemia/reperfusion injury
文献摘要：
Glaucoma results from irreversible loss of retinal ganglion cells(RGCs)through an unclear mechanism.Microglial polarization and neuroinflammation play an important role in retinal degeneration.Our study aimed to explore the function of microglial polarization during glaucoma progression and identify a strategy to alleviate retinal neuroinflammation.Retinal ischemia/reperfusion injury was induced in C57BL/6 mice.In a separate cohort of animals,interleukin(IL)-4(50 ng/mL,2 pL per injection)or vehicle was intravitreally injected after retinal ischemia/reperfusion injury.RGC loss was assessed by counting cells that were positive for the RGC marker RNA binding protein,mRNA processing factor in retinal flat mounts.The expression of classically activated(M1)and alternatively activated(M2)microglial markers were assessed by quantitative reverse transcription-polymerase chain reaction,immunofluorescence,and western blotting.The results showed that progressive RGC loss was accompanied by a continuous decrease in M2 microglia during the late phase of the 28-day period after retinal ischemia/reperfusion injury.IL-4 was undetectable in the retina at all time points,and intravitreal IL-4 administration markedly improved M2 microglial marker expression and ameliorated RGC loss in the late phase post-retinal ischemia/reperfusion injury.In summary,we observed that IL-4 treatment maintained a high number of M2 microglia after RIR and promoted RGC survival.
文献关键词：
中图分类号：
作者姓名：
Di Chen;Cheng Peng;Xu-Ming Ding;Yue Wu;Chang-Juan Zeng;Li Xu;Wen-Yi Guo
作者机构：
Department of Ophthalmology,Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China;Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology,Shanghai,China
文献出处：
引用格式：
[1]Di Chen;Cheng Peng;Xu-Ming Ding;Yue Wu;Chang-Juan Zeng;Li Xu;Wen-Yi Guo-.Interleukin-4 promotes microglial polarization toward a neuroprotective phenotype after retinal ischemia/reperfusion injury)[J].中国神经再生研究（英文版）,2022(12):2755-2760
A类：
intravitreally,mounts
B类：
Interleukin,promotes,microglial,polarization,toward,neuroprotective,phenotype,after,retinal,ischemia,reperfusion,injury,Glaucoma,results,from,irreversible,loss,ganglion,cells,RGCs,through,unclear,mechanism,Microglial,neuroinflammation,play,important,role,degeneration,Our,study,aimed,explore,function,during,glaucoma,progression,identify,strategy,alleviate,Retinal,was,induced,C57BL,mice,separate,cohort,animals,interleukin,pL,injection,vehicle,injected,assessed,by,counting,that,were,positive,binding,protein,processing,flat,expression,classically,activated,M1,alternatively,M2,markers,quantitative,reverse,transcription,polymerase,chain,reaction,immunofluorescence,western,blotting,showed,progressive,accompanied,continuous,decrease,late,phase,day,period,undetectable,points,administration,markedly,improved,ameliorated,post,summary,observed,treatment,maintained,high,number,RIR,promoted,survival
AB值：
0.529254
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
