OBJECTIVE:To explore the effect and mechanism of baicalin in the treatment of acute lung injury(ALI)by in vivo and in vitro experiments.METHODS:ALI was induced by instilling 10 mg/mL lipopolysaccharide(LPS)into the airway of rats.Different doses of baicalin(50 and 100 mg·kg-1·d-1)were administered by gavage one day before modeling.RESULTS:Baicalin significantly reduced the permeability of the alveolocapillary membrane,alleviated tissue injury and inflammatory infiltration,and inhibited the secretion of inflammatory factors and the infiltration of neutrophils.The decline in these inflammations was related to the inhibition of the toll like receptor-4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear factor-kappa B(NF-KB)/nod-like receptor pyrin containing 3(NLRP3)signaling pathway and the mitogen-activated protein kinase(MAPK)signaling pathway.CONCLUSIONS:Baicalin inhibits the secretion of inflammatory factors by inhibiting the TLR4-MyD88-NF-κB/NLRP3 pathway and the MAPK signaling pathway.Thus,it reduces lung bronchial epithelial layer,alveolar damage,and pulmonary edema as detected in the in vivo and in vitro experiments.Therefore,baicalin may be a potential preventive and therapeutic drug for ALI.

ZHU Changle;FENG Cuiling;FENG Feng;Yao Xiaoqin;WANG Guishu;SHI Liangtian;ZHENG Jiakun

Department of respiration,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100000,China;Department of traditional Chinese Medicine,Peking University People's Hospital,Beijing,100000,China;Department of Traditional Chinese Medicine,Peking University International Hospital,Beijing 100000,China

中医杂志（英文版）

[1]ZHU Changle;FENG Cuiling;FENG Feng;Yao Xiaoqin;WANG Guishu;SHI Liangtian;ZHENG Jiakun-.Baicalin inhibits inflammation of lipopolysaccharide-induced acute lung injury via toll like receptor-4/myeloid differentiation primary response 88/nuclear factor-kappa B signaling pathway)[J].中医杂志（英文版）,2022(02):200-212

instilling,alveolocapillary,inflammations

Baicalin,inhibits,lipopolysaccharide,induced,acute,lung,injury,toll,like,receptor,myeloid,differentiation,primary,response,nuclear,kappa,signaling,pathway,OBJECTIVE,To,explore,effect,mechanism,baicalin,treatment,ALI,by,vivo,vitro,experiments,METHODS,was,LPS,into,airway,rats,Different,doses,were,administered,gavage,one,day,before,modeling,RESULTS,significantly,reduced,permeability,membrane,alleviated,tissue,inflammatory,infiltration,inhibited,secretion,factors,neutrophils,decline,these,related,inhibition,TLR4,MyD88,KB,nod,pyrin,containing,NLRP3,mitogen,activated,protein,kinase,MAPK,CONCLUSIONS,inhibiting,Thus,reduces,bronchial,epithelial,layer,alveolar,damage,pulmonary,edema,detected,Therefore,may,potential,preventive,therapeutic,drug

0.546033