典型文献
Seminal HPV detection:a pilot study comparing the preservation effectiveness and cost between a methanol-based solution and cryopreservation with liquid nitrogen
文献摘要：
Dear Editor,
Human papillomavirus(HPV)is the most common sexually transmitted virus worldwide,and high-risk HPV infection is the most important risk factor for cervical cancer.1 However,few conclusive data are available on the prevalence of the male HPV infection and its health consequences.It has been found that the prevalence of seminal HPV infection is higher in men with unexplained infertility,ranging between 10％and 35％compared with the general population(range:2％to 11％).2-4 In three recent systematic reviews,it was seen that HPV sperm infection may be associated with impaired sperm function that could lead to male infertility.2,4,5 HPV sperm infection with high-risk HPV among infertile men was shown to be associated with lower progressive sperm motility and higher DNA fragmentation index.6 Moreover,a high rate of pregnancy loss has been found among couples with HPV sperm infection after in vitro fertilization(IVF).7 In the majority of the studies performed to assess seminal HPV infection,samples were preserved in liquid nitrogen until analysis with polymerase chain reaction(PCR).Liquid nitrogen is used to store cells at low temperatures;however,its handling and laboratory safety requires special infrastructure.An alternative,not requiring cryopreservation,is the methanol-based ThinPrep PreservCyt Solution(Hologic,Marlborough,MA,USA).It is a low-cost and safe reagent that serves as a collection,transport,and preservative medium widely used in Sweden for cervical HPV detection.
文献关键词：
中图分类号：
作者姓名：
Jynfiaf Francis;Cecilia K?rrberg;Jonas Hermansson;Magnus Lindh;Sofia Ganidou;Ann Thurin-Kjellberg;Kersti Lundin;Randa Akouri;Panagiotis Tsiartas
作者机构：
Department of Reproductive Medicine,Sahlgrenska University Hospital,Gothenburg 413 45,Sweden;Department of Obstetrics and Gynecology,Institute of Clinical Sciences,Sahlgrenska Academy,Gothenburg University,Gothenburg 405 30,Sweden;Department of Research and Development,Angered Hospital,Angered 424 65,Sweden;Department of Clinical Microbiology,Sahlgrenska University Hospital,Gothenburg 413 46,Sweden;Department of Oncology-Pathology,Karolinska Institute,Stockholm 171 77,Sweden
文献出处：
引用格式：
[1]Jynfiaf Francis;Cecilia K?rrberg;Jonas Hermansson;Magnus Lindh;Sofia Ganidou;Ann Thurin-Kjellberg;Kersti Lundin;Randa Akouri;Panagiotis Tsiartas-.Seminal HPV detection:a pilot study comparing the preservation effectiveness and cost between a methanol-based solution and cryopreservation with liquid nitrogen)[J].亚洲男性学杂志（英文版）,2022(05):560-561
A类：
PreservCyt,Marlborough
B类：
Seminal,HPV,detection,pilot,study,comparing,effectiveness,cost,between,methanol,solution,cryopreservation,liquid,nitrogen,Dear,Editor,
Human,papillomavirus,most,common,sexually,transmitted,worldwide,risk,infection,important,cervical,cancer,However,few,conclusive,data,available,prevalence,male,its,health,consequences,It,has,been,found,that,seminal,higher,unexplained,infertility,ranging,compared,general,population,range,In,three,recent,systematic,reviews,was,seen,sperm,may,associated,impaired,function,could,lead,among,infertile,shown,lower,progressive,motility,fragmentation,Moreover,rate,pregnancy,loss,couples,after,vitro,fertilization,IVF,majority,studies,performed,assess,samples,were,preserved,until,analysis,polymerase,chain,reaction,Liquid,used,store,cells,temperatures,however,handling,laboratory,safety,requires,special,infrastructure,An,alternative,not,requiring,ThinPrep,Solution,Hologic,MA,USA,reagent,serves,collection,transport,preservative,medium,widely,Sweden
AB值：
0.582961
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