典型文献
Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation
文献摘要：
Understanding the regulatory networks for germ cell fate specification is necessary to developing strategies for improving the efficiency of germ cell production in vitro.In this study,we developed a coupled screening strategy that took advantage of an arrayed bi-molecular fluorescence complementation(BiFC)platform for pro-tein-protein interaction screens and epiblast-like cell(EpiLC)-induction assays using reporter mouse embry-onic stem cells(mESCs).Investigation of candidate interaction partners of core human pluripotent factors OCT4,NANOG,KLF4 and SOX2 in EpiLC differentiation assays identified novel primordial germ cell(PGC)-in-ducing factors including BEN-domain(BEND/Bend)family members.Through RNA-seq,ChlP-seq,and ATAC-seq analyses,we showed that Bend5 worked together with Bend4 and helped mark chromatin boundaries to promote EpiLC induction in vitro.Our findings suggest that BEND/Bend proteins represent a new family of transcriptional modulators and chromatin boundary factors that participate in gene expression regulation during early germline development.
文献关键词：
中图分类号：
作者姓名：
Guang Shi;Yaofu Bai;Xiya Zhang;Junfeng Su;Junjie Pang;Quanyuan He;Pengguihang Zeng;Junjun Ding;Yuanyan Xiong;Jingran Zhang;Jingwen Wang;Dan Liu;Wenbin Ma;Junjiu Huang;Zhou Songyang
作者机构：
MOE Key Laboratory of Gene Function and Regulation,Guangzhou Key Laboratory of Healthy Aging Research,School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China;Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;Center for Reproductive Medicine,The Third Affiliated Hospital of Sun Yat-sen University,Sun Yat-sen University,Guangzhou 510630,China;Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou 510080,China;Verna and Marrs Mclean Department of Biochemistry and Molecular Biology,Baylor College of Medicine,One Baylor Plaza,Houston,TX 77030,USA;Bioland Laboratory,Guangzhou 510320,China
文献出处：
引用格式：
[1]Guang Shi;Yaofu Bai;Xiya Zhang;Junfeng Su;Junjie Pang;Quanyuan He;Pengguihang Zeng;Junjun Ding;Yuanyan Xiong;Jingran Zhang;Jingwen Wang;Dan Liu;Wenbin Ma;Junjiu Huang;Zhou Songyang-.Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation)[J].蛋白质与细胞,2022(10):721-741
A类：
Bend,EpiLC,embry,mESCs,BEND,ChlP,Bend5,Bend4
B类：
family,proteins,mark,chromatin,boundaries,synergistically,promote,early,differentiation,Understanding,regulatory,networks,fate,specification,necessary,developing,strategies,improving,efficiency,production,vitro,this,study,developed,coupled,screening,strategy,that,took,advantage,arrayed,bi,molecular,fluorescence,complementation,BiFC,platform,interaction,screens,epiblast,like,induction,assays,using,reporter,mouse,onic,stem,cells,Investigation,candidate,partners,core,human,pluripotent,factors,OCT4,NANOG,KLF4,SOX2,identified,novel,primordial,PGC,ducing,including,domain,members,Through,seq,ATAC,analyses,showed,worked,together,helped,Our,findings,suggest,represent,new,transcriptional,modulators,boundary,participate,gene,expression,regulation,during,germline,development
AB值：
0.568224
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
