典型文献
Evolution of the ErbB gene family and analysis of regulators of Egfr expression during development of the rat spinal cord
文献摘要：
Egfr, a member of the ErbB gene family, plays a critical role in tissue development and homeostasis, wound healing, and disease. However, expression and regulators of Egfr during spinal cord development remain poorly understood. In this study, we investigated ErbB evolution and analyzed co-expression modules, miRNAs, and transcription factors that may regulate Egfr expression in rats. We found that ErbB family members formed via Egfr duplication in the ancient vertebrates but diverged after speciation of gnathostomes. We identified a module that was co-expressed with Egfr, which involved cell proliferation and blood vessel development. We predicted 25 miRNAs and nine transcription factors that may regulate Egfr expression. Dual-luciferase reporter assays showed six out of nine transcription factors significantly affected Egfr promoter reporter activity. Two of these transcription factors (KLF1 and STAT3) inhibited the Egfr promoter reporter, whereas four transcription factors (including FOXA2) activated the Egfr promoter reporter. Real-time PCR and immunofluorescence experiments showed high expression of FOXA2 during the embryonic period and FOXA2 was expressed in the floor plate of the spinal cord, suggesting the importance of FOXA2 during embryonic spinal cord development. Considering the importance of Egfr in embryonic spinal cord development, wound healing, and disease (specifically in cancer), regulatory elements identified in this study may provide candidate targets for nerve regeneration and disease treatment in the future.
文献关键词：
中图分类号：
作者姓名：
Yu Zhang;Tao Zhang;Lian Xu;Ye Zhu;Li-Li Zhao;Xiao-Di Li;Wei-Wei Yang;Jing Chen;Miao Gu;Xiao-Song Gu;Jian Yang
作者机构：
School of Medicine&Holistic Integrative Medicine,Nanjing University of Chinese Medicine,Nanjing,Jiangsu Province,China;Key Laboratory of Neuroregeneration of JiangsuProvince and Ministry of Education,Co-innovation Center of Neuroregeneration,NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products,Nantong University,Nantong,Jiangsu Province,China
文献出处：
引用格式：
[1]Yu Zhang;Tao Zhang;Lian Xu;Ye Zhu;Li-Li Zhao;Xiao-Di Li;Wei-Wei Yang;Jing Chen;Miao Gu;Xiao-Song Gu;Jian Yang-.Evolution of the ErbB gene family and analysis of regulators of Egfr expression during development of the rat spinal cord)[J].中国神经再生研究（英文版）,2022(11):2484-2490
A类：
gnathostomes
B类：
Evolution,ErbB,family,analysis,regulators,Egfr,expression,during,development,spinal,cord,plays,critical,role,tissue,homeostasis,wound,healing,disease,However,remain,poorly,understood,In,this,study,investigated,evolution,analyzed,modules,miRNAs,transcription,factors,that,may,regulate,rats,We,found,members,formed,via,duplication,ancient,vertebrates,but,diverged,after,speciation,identified,was,expressed,which,involved,cell,proliferation,blood,vessel,predicted,nine,Dual,luciferase,reporter,assays,showed,six,out,significantly,affected,promoter,activity,Two,these,KLF1,STAT3,inhibited,whereas,four,including,FOXA2,activated,Real,immunofluorescence,experiments,high,embryonic,period,floor,plate,suggesting,importance,Considering,specifically,cancer,regulatory,elements,provide,candidate,targets,nerve,regeneration,treatment,future
AB值：
0.472994
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
