Formaldehyde(FA)plays critical roles in Alzheimer's disease and the associations between FA and Alzheimer's disease(AD)are still obscure.To reveal FA fluxes in the Alzheimer's disease brain,an activity-based fluorescence probe NP-FA with superb blood-brain barrier permeable abilities was exquisitely designed.The probe responded to FA with significant fluorescence increases(F/F0=81),thus laying the foundation for the sensitive detection of FA in cuvette and in vivo.Moreover,the probe also possessed some fasci-nating performances,including endoplasmic reticulum(ER)-targeting abilities,good one-photon/two-photon absorption properties,and appropriate hydrophobicity property(log P=2.34±0.05).As a result,the probe can readily reflect the overproduction of FA con-tent in live cells under ER stress by high-fidelity two-photon imaging.More interestingly,ex vivo imaging of AD brains and two-photon imaging of AD slice tissues visually disclosed that the FA level of AD brain is much higher than that of the normal brain.This work afforded a specific activity-based probe for the imaging of FA in the AD mouse brains,which could be further extended to FA-related studies in Alzheimer's disease.

Pengzhan Wang;Xianhua Cheng;Jianhua Xiong;Zhiqiang Mao;Zhihong Liu

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials,Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules&College of Chemistry and Chemical Engineering,Hubei University,Wuhan,Hubei 430062,China;College of Chemistry and Molecular Sciences,Wuhan University,Wuhan,Hubei 430072,China

中国化学（英文版）

[1]Pengzhan Wang;Xianhua Cheng;Jianhua Xiong;Zhiqiang Mao;Zhihong Liu-.Revealing Formaldehyde Fluxes in Alzheimer's Disease Brain by an Activity-Based Fluorescence Probe)[J].中国化学（英文版）,2022(12):1457-1463

cuvette

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