典型文献
Improvement in Resolution of Multiphoton Scanning Structured Illumination Microscopy via Harmonics
文献摘要：
We describe a multiphoton(mP)-structured illumination microscopy(SIM)technique,which demon-strates substantial improvement in image resolution compared with linear SIM due to the nonlinear response of fluorescence.This nonlinear response is caused by the effect of nonsinusoidal structured illu-mination created by scanning a sinusoidally modulated illumination to excite an mP fluorescence signal.The harmonics of the structured fluorescence illumination are utilised to improve resolution.We present an mP-SIM theory for reconstructing the super-resolution image of the system.Theoretically,the resolution of our mP-SIM is unlimited if all the high-order harmonics of the nonlinear response of fluorescence are con-sidered.Experimentally,we demonstrate an 86 nm lateral resolution for two-photon(2P)-SIM and a 72 nm lateral resolution for second-harmonic-generation(SHG)-SIM.We further demonstrate their application by imaging cells stained with F-actin and collagen fibres in mouse-tail tendon.Our method can be directly used in commercial mP microscopes and requires no specific fluorophores or high-intensity laser.
文献关键词：
中图分类号：
作者姓名：
Lei Wang;Xiaomin Zheng;Jie Zhou;Meiting Wang;Jiajie Chen;Youjun Zeng;Gaixia Xu;Ying Wang;Haixia Qiu;Yonghong Shao;Junle Qu;Bruce Zhi Gao;Ying Gu
作者机构：
College of Physics and Optoelectronic Engineering,Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,Shenzhen University,Shenzhen 518060,China;Department of Laser Medicine,First Medical Center of PLA General Hospital,Beijing 100853,China;Department of Bioengineering and COMSET,Clemson University,Clemson,SC 29634,USA
文献出处：
引用格式：
[1]Lei Wang;Xiaomin Zheng;Jie Zhou;Meiting Wang;Jiajie Chen;Youjun Zeng;Gaixia Xu;Ying Wang;Haixia Qiu;Yonghong Shao;Junle Qu;Bruce Zhi Gao;Ying Gu-.Improvement in Resolution of Multiphoton Scanning Structured Illumination Microscopy via Harmonics)[J].工程（英文）,2022(09):65-72
A类：
Harmonics,nonsinusoidal
B类：
Improvement,Resolution,Multiphoton,Scanning,Structured,Illumination,Microscopy,via,We,describe,multiphoton,mP,structured,illumination,microscopy,SIM,technique,which,strates,substantial,improvement,image,resolution,compared,due,nonlinear,response,fluorescence,This,caused,by,effect,created,scanning,sinusoidally,modulated,excite,signal,harmonics,utilised,present,theory,reconstructing,super,system,Theoretically,our,unlimited,high,order,sidered,Experimentally,we,demonstrate,lateral,two,2P,second,generation,SHG,further,their,application,imaging,cells,stained,actin,collagen,fibres,mouse,tail,tendon,Our,method,directly,commercial,microscopes,requires,specific,fluorophores,intensity,laser
AB值：
0.545369
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
