典型文献
Circulating Levels of Hypoxia-regulating MicroRNAs in Systemic Lupus Erythematosus Patients with Hemolytic Anemia
文献摘要：
Objective:MicroRNAs are fine regulators for gene expression during the post-transcriptional stage in many autoimmune diseases.HypoxamiRs(miR-210 and miR-21)play an important role in hypoxia and in inflammation-associated hypoxia.Systemic lupus erythematosus(SLE)is a chronic systemic autoimmune disease that would potentiate many pathological complications,including hemolytic anemia.This study aimed to investigate the role of hypoxamiRs in SLE/hemolytic anemia patients.Methods:This work was designed to analyze the circulating levels of the miR-210 and miR-21 expressions and hypoxia-inducible factor-1α(HIF-α)in SLE/hemolytic anemia patients.SLE activity was evaluated for all patients by SLE Disease Activity Index(SLEDAI).Clinical manifestations/complications and serological/hematological investigations were reported.HIF-α concentration was assayed by ELISA and expression of miR-21 and miR-210 was analyzed by qRT-PCR.Results:The results indicated that the fold change of the miR-210/miR-21 expressions in plasma was significantly elevated in SLE/hemolytic anemia patients.A strong positive correlation between the miR-210 and miR-21 expression levels was also recorded.Among the associated-disease complications,hypertension,arthritis,oral ulcers,and serositis were associated with a high circulating miR-210 expression,while the occurrence of renal disorders was associated with the increased miR-21 expression.Furthermore,the HIF-αlevel was remarkably elevated in SLE/hemolytic anemia patients.A high positive correlation was recorded between the HIF-α concentration and miR-210/miR-21 expression levels.The occurrence of oral ulcers,arthritis,and hypertension was associated with the increased HIF-α concentration.On the other hand,SLEDAI and white blood cell count were positively correlated with miR-21/miR-210.The erythrocyte sedimentation rate was positively correlated with miR-21.Conclusion:The dysregulation of the circulating miR-210/miR-210/HIF-1α levels in SLE/hemolytic anemia patients advocated that the hypoxia pathway might have an essential role in the pathogenesis and complications of these diseases.
文献关键词：
中图分类号：
作者姓名：
Amira M.Gamal-Eldeen;Cinderella A.Fahmy;Bassem M.Raafat;Fayez Althobaiti;Iman H.Bassyouni;Roba M.Talaat
作者机构：
Clinical Laboratory Sciences Department,College of Applied Medical Sciences,Taif University,Taif 21944,Saudi Arabia;High Altitude Research Center,Prince Sultan Medical Complex,Al-Hawiyah,Taif University,Taif 21944,Saudi Arabia;Cancer Biology and Genetics Laboratory,Centre of Excellence for Advanced Sciences,National Research Centre,Cairo 12622,Egypt;Biochemistry Department,National Research Centre,Cairo 12622,Egypt;Radiological Sciences Department,College of Applied Medical Sciences,Taif University,Taif 21944,Saudi Arabia;Biotechnology Department,Faculty of Science,Taif University,Taif 21944,Saudi Arabia;Department of Rheumatology and Rehabilitation,Faculty of Medicine,Cairo University,El-Kasr El-Aini Hospital,Cairo 12613,Egypt;Molecular Biology Department,Genetic Engineering and Biotechnology Research Institute(GEBRI),Sadat City University,Sadat City 32897,Egypt
文献出处：
引用格式：
[1]Amira M.Gamal-Eldeen;Cinderella A.Fahmy;Bassem M.Raafat;Fayez Althobaiti;Iman H.Bassyouni;Roba M.Talaat-.Circulating Levels of Hypoxia-regulating MicroRNAs in Systemic Lupus Erythematosus Patients with Hemolytic Anemia)[J].当代医学科学(英文),2022(06):1231-1239
A类：
HypoxamiRs,hypoxamiRs,serositis
B类：
Circulating,Levels,Hypoxia,regulating,MicroRNAs,Systemic,Lupus,Erythematosus,Patients,Hemolytic,Anemia,Objective,are,fine,regulators,during,post,transcriptional,stage,many,autoimmune,diseases,play,important,role,hypoxia,inflammation,associated,lupus,erythematosus,chronic,systemic,that,would,potentiate,pathological,complications,including,hemolytic,anemia,This,study,aimed,investigate,patients,Methods,work,was,designed,circulating,levels,expressions,inducible,HIF,activity,evaluated,all,by,Disease,Activity,Index,SLEDAI,Clinical,manifestations,serological,hematological,investigations,were,reported,concentration,assayed,analyzed,qRT,Results,results,indicated,fold,change,plasma,significantly,elevated,strong,correlation,between,also,recorded,Among,hypertension,arthritis,oral,ulcers,high,while,occurrence,renal,disorders,increased,Furthermore,remarkably,On,other,hand,white,blood,cell,count,positively,correlated,erythrocyte,sedimentation,rate,Conclusion,dysregulation,advocated,pathway,might,have,essential,pathogenesis,these
AB值：
0.464227
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