Commercial tissue adhesives have been widely applied in wound hemostats and dressings while enhancing the hemostasis and healing capabilities is challenging to meet clinical needs.Herein,we designed the glucose-and catechol-functionalized derivatives from commercial ε-polylysine(EPL)and prepared the hydrogels by simple amidation and catechol-crosslinking reactions,which have larger swelling ratios of 220％-240％,suitable microporous size of about 6-8 μm,and tissue adhesion strength of about 20-40 kPa.The hemolysis,cytotoxicity,and cellular double-staining assays indicate that those hydrogels had good biocompatibility and the H-3 hydrogel with higher glucose content gave a lower hemolysis ratio of 0.73％±0.14％.The blood-clotting index,blood cell attachment and adhesion studies showed those hydrogels had fast blood-coagulation,resulting in excellent hemostasis performance with a short hemostatic time of 38-46 sand less blood loss of 19％-34％in a liver hemorrhage model.A full-thickness rat-skin defect model further demonstrates that the H-3 hydrogel achieved fast wound healing with a wound closure of 70.0％±2.7％on postoperative day 7 and nearly full closure on day 14.Remarkably,the hydroproline level that denotes the collagen production reached a higher one of 7.24±0.55 μg/mg comparable to that in normal skins on day 14,evidencing the wound healing was close to completion in the H-3 treatment.Consequently,this work provides a simple method to construct a glycosylated and catechol-functionalized hydrogel platform from commercial EPL,holding translational potentials in wound hemostats and dressings.

Lin Teng;Zheng-Wei Shao;Yu-Shi He;Jia-Yu Lu;De-Rong Zou;Chuan-Liang Feng;Chang-Ming Dong

School of Chemistry and Chemical Engineering,Frontiers Science Center for Transformative Molecules,Shanghai Key Laboratory of Electrical Insulation and Thermal Aging,Shanghai Jiao Tong University,Shanghai200240,China;Department of Stomatology,Shanghai Jiao Tong University Affiliated Sixth People's Hospital,Shanghai Jiao Tong University,Shanghai 200233,China;School of Chemistry and Chemical Engineering,Shanghai Electrochemical Energy Devices Research Center,Shanghai Jiao Tong University,Shanghai 200240,China;School of Materials Science and Engineering,State Key Laboratory of Metal Matrix Composites,Shanghai Jiao Tong University,Shanghai200240,China

高分子科学（英文版）

[1]Lin Teng;Zheng-Wei Shao;Yu-Shi He;Jia-Yu Lu;De-Rong Zou;Chuan-Liang Feng;Chang-Ming Dong-.A Glycosylated and Catechol-crosslinked ε-Polylysine Hydrogel:Simple Preparation and Excellent Wound Hemostasis and Healing Properties)[J].高分子科学（英文版）,2022(09):1110-1119

Polylysine,Hemostasis,hemostats,amidation,hydroproline

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