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典型文献
Integrated Organ-on-a-chip with Human-induced Pluripotent Stem Cells Directional Differentiation for 3D Skin Model Generation
文献摘要:
Keratinocytes and fibroblasts, derived from hiPSCs, were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid) (PLGA) nanofibers and a human skin-on-a-chip device. Unlike the conventional culture dish method, two different epidermal cells are successfully adhered to the front and back sides of the patch, which produces a three-dimensional nanofibrous scaffold similar to a natural extracellular matrix before the patch was cultured in the skin-on-a-chip device to mimic the physiological conditions of human skin. As expected, the differentiated hiPSCs show the expression of keratinocyte- and fibroblast- specific proteins on the patch, and the layering is found between these two kinds of cells, indicating that this approach creates a powerful in vitro system for modeling skin development and diseases.
文献关键词:
作者姓名:
LUO Zhang;GONG Chaihong;MAO Xiaowei;WANG Zhe;LIU Zhifan;BEN Yali;ZHANG Weiying
作者机构:
School of Life Sciences,Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education,Jianghan Universi-ty,Wuhan 430056,China;School of Environment and Health,Jiang-han University,Wuhan 430056,China;School of medicine,Jianghan University,Wuhan 430056,China
引用格式:
[1]LUO Zhang;GONG Chaihong;MAO Xiaowei;WANG Zhe;LIU Zhifan;BEN Yali;ZHANG Weiying-.Integrated Organ-on-a-chip with Human-induced Pluripotent Stem Cells Directional Differentiation for 3D Skin Model Generation)[J].武汉理工大学学报(材料科学版)(英文版),2022(06):1271-1278
A类:
Keratinocytes
B类:
Integrated,Organ,chip,Human,induced,Pluripotent,Stem,Cells,Directional,Differentiation,Skin,Model,Generation,fibroblasts,derived,from,hiPSCs,were,used,construct,human,epidermal,by,patch,made,monolayer,poly,lactic,glycolic,acid,PLGA,nanofibers,skin,device,Unlike,conventional,dish,method,two,cells,are,successfully,adhered,front,back,sides,which,produces,three,dimensional,nanofibrous,scaffold,similar,natural,extracellular,matrix,before,was,cultured,mimic,physiological,conditions,expected,differentiated,show,expression,keratinocyte,specific,proteins,layering,found,between,these,kinds,indicating,that,this,approach,creates,powerful,vitro,system,modeling,development,diseases
AB值:
0.682332
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