典型文献
Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish
文献摘要：
Vibrio parahaemolyticus is the main cause of foodborne gastroenteritis,which is widely distributed in shellfish and other seafood.Most V parahaemolyticus are nonpathogenic,and only a few types,such as serotype O3:K6,are pathogenic,which is also the most prevalent strain in Asia.However,the relationship between this serotype and pathogenicity has yet to be established.The waaf gene is located in the O antigen synthesis gene cluster.Thus,we constructed a waaf gene deletion mutant(i.e.,△waaf)of wild-type(WT)which isolated from shellfish serotype O3:K6 via chitin-mediated transformation technology.We then constructed the △waaf complementary strain(i.e.,C-△waaf)via the Escherichia coli S17 λpir strain by conjugation.The basic physiological characteristics,adhesion to Caco2 cells,and pathogenicity of the WT,△waaf,and C-△waaf strains were compared.Growth curves showed no remarkable differences between the WT and △waaf strains.However,the △waaf strain non-reactive to O3 antisera and other 12 O-group antisera of V parahaemolyticus.Moreover,the number of flagella and extracellular polysaccharides decreased,the adhesion decreased,and the pathogenicity weakened.These characteristics of the C-△waaf strain were similar to those of the WT strain.These results indicated that the waaf gene is vital to the serotype in V parahaemolyticus,and changes in O antigen could affect the pathogenicity of this bacterium.This study will be helpful to understand the pathogenic mechanism of V parahaemolyticus.
文献关键词：
中图分类号：
作者姓名：
Feng Zhao;Guoying Ding;Qilong Wang;Huihui Du;Guosheng Xiao;Deqing Zhou
作者机构：
College of biology and food engineering,Chongqing Three Gorges University,Wanzhou 404100,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266071,China
文献出处：
引用格式：
[1]Feng Zhao;Guoying Ding;Qilong Wang;Huihui Du;Guosheng Xiao;Deqing Zhou-.Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish)[J].食品科学与人类健康（英文）,2022(02):418-426
A类：
waaf,nonpathogenic,antisera
B类：
Deletion,gene,affects,antigen,synthesis,pathogenicity,Vibrio,parahaemolyticus,from,shellfish,main,cause,foodborne,gastroenteritis,which,widely,distributed,other,seafood,Most,only,few,types,such,serotype,O3,K6,also,most,prevalent,Asia,However,relationship,between,this,has,yet,established,located,cluster,Thus,constructed,deletion,mutant,wild,WT,isolated,via,chitin,mediated,transformation,technology,We,then,complementary,Escherichia,coli,S17,pir,by,conjugation,basic,physiological,characteristics,adhesion,Caco2,cells,strains,were,compared,Growth,curves,showed,remarkable,differences,reactive,group,Moreover,number,flagella,extracellular,polysaccharides,decreased,weakened,These,similar,those,results,indicated,that,vital,changes,could,bacterium,This,study,will,helpful,understand,mechanism
AB值：
0.458061
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