Background : Experimental animals are used to study physiological phenomena, pathological mechanisms, and disease prevention. The gut microbiome is known as a potential confounding factor for inconsistent data from preclinical studies. Although many gut microbiome studies have been conducted in recent decades, few have fo-cused on gut microbiota fluctuation among representative mouse strains. Methods : A range of frequently used mouse strains were selected from 34 isolation packages representing disease- related animal (DRA), immunity defect animal (IDA), or gene- editing animal (GEA) from the BALB/c and C57BL/6J backgrounds together with normal mice, and their microbial genomic DNA were isolated from mouse feces to sequence for the exploration of gut microbiota. Results : Mouse background strain, classification, introduced source, introduced year, and reproduction type significantly affected the gut microbiota structure ( p < 0.001 for all parameters), with background strain contributing the greatest influence ( R 2 = 0.237). In normal groups, distinct gut microbiota types existed in different mouse strains. Sixty- four core operational taxonomic units were obtained from normal mice, and 12 belonged to Lactobacillus. Interestingly, the gut microbiota in C57BL/6J was more stable than that in BALB/c mice. Furthermore, the gut microbiota in the IDA, GEA, and DRA groups significantly differed from that in normal groups ( p < 0.001 for all). Compared with the normal group, there was a significantly higher Chao1 and Shannon index ( p < 0.001 for all) in the IDA, GEA, and DRA groups. Markedly changed classes occurred with Firmicutes and Bacteroidetes . The abundances of Helicobacter , Blautia , Enterobacter , Bacillus , Clostridioides , Paenibacillus , and Clostridiales all signifi-cantly decreased in the IDA, GEA, and DRA groups, whereas those of Saccharimonas , Rikenella , and Odoribacter all significantly increased.

Jianguo Guo;Chenchen Song;Yunbo Liu;Xuying Wu;Wei Dong;Hua Zhu;Zhiguang Xiang;Chuan Qin

Key Laboratory of Human Disease Comparative Medicine,Chinese Ministry of Health,Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases,Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences and Comparative Medicine Center,Peking Union Medical College,Beijing,China

动物模型与实验医学（英文）

[1]Jianguo Guo;Chenchen Song;Yunbo Liu;Xuying Wu;Wei Dong;Hua Zhu;Zhiguang Xiang;Chuan Qin-.Characteristics of gut microbiota in representative mice strains: Implications for biological research)[J].动物模型与实验医学（英文）,2022(04):337-349

cused,Markedly

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