Helicobacter pylori(H.pylori)is known to be a major risk factor for the development of gastric cancer.In recent years,increasing attention is being paid to the role of non-H.pylori Helicobacters(NHPHs)in this disease and the role of microorganisms in local tumor microenvironment.In this study,we aimed to compare the microbial com-munity composition and the predicted functional profile in paired cancer and adjacent normal tissues of gastric cancer patients.Cancer tissues and adjacent normal tissues were collected from 10 patients with gastric cancer under endoscopy,and genomic DNA was extracted.The V3-V4 region of the 16S rRNA gene was amplified by PCR and paired-end sequencing was performed on the lllumina MiSeq System.The data was analyzed using QIIME 2 software.The results showed that microbial richness and diversity as well as genetic diversity are significantly lower in cancer tissues compared with adjacent normal tissues.At the phylum level,the dominant taxa are Pro-teobacteria,Thermi,Actinobacteria,Bacteroidetes and Firmicutes in both groups.At the genus level,some taxa,such as Cupriavidus and Sphingomonas,are significantly enriched in cancer tissues,while other taxa,such as Ochrobactrum,are enriched in adjacent normal tissues.Moreover,those taxa enriched in cancer tissues are as-sociated with the synthesis and degradation of ketone bodies.In conclusion,there is a significant difference in the composition of the mucosa-related microbial communities between cancer tissues and adjacent normal tissues in patients with gastric cancer.

Linli Shi;Qilin Fan;Bin Zhou;Jingjing Wu;Min Jin;Dandan Yu;Tao Zhang;Jun Song;Hongli Liu

Cancer Center,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Gastroenterology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

生物化学与生物物理学报（英文版）

[1]Linli Shi;Qilin Fan;Bin Zhou;Jingjing Wu;Min Jin;Dandan Yu;Tao Zhang;Jun Song;Hongli Liu-.The composition and functional profile of the microbial communities in human gastric cancer tissues and adjacent normal tissues)[J].生物化学与生物物理学报（英文版）,2022(01):47-54

Helicobacters,NHPHs,Thermi

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