Background:Increasing demand for high-value fish species and pressure on forage fish is challenging aquaculture to ensure sustainable growth by replacing protein sources in aquafeeds with plant and terrestrial animal proteins, without compromising the economic value and quality of the final fish product. In the present study, the effects of a plant protein-based diet (CV), two plant-based diets in which graded amounts of plan protein mixtures were replaced with Hermetia illucens meal alone (VH10) or in combination with poultry by-product meal (PBM) (VH10P30), a fishmeal (FM) diet (CF) and an FM diet supplemented with H. illucens (FH10) on growth performance, gut health and homeostasis of farmed subadult European seabass were tested and compared. Results:Fish fed the VH10 and VH10P30 diets showed the highest specific growth rates and lowest feed conversion ratios among the tested groups. Expectedly, the best preservation of PI morphology was observed in fish fed the CF or FH10 diets, while fish fed the CV diet exhibited significant degenerative changes in the proximal and distal intestines. However, PBM supplementation mitigated these effects and significantly improved all gut morphometric parameters in the VH10P30 group. Partial substitution of the plant mixture with insect meal alone or PBM also induced most BBM genes and activated BBM enzymes, suggesting a beneficial effect on intestinal digestive/absorption functions. Regarding intestinal microbiota, fish fed diets containing H. illucens meal (FH10, VH10, VH10P30) had the highest richness of bacterial communities and abundance of beneficial genera such as Lactobacillus and Bacillus. On the other hand, fish fed CV had the highest microbial diversity but lost a significant component of fish intestinal microbiota, the phylum Bacteroidetes. Finally, skin pigmentation most similar to that of farmed or even wild seabass was also observed in the fish groups fed CF, FH10 or VH10P30. Conclusion:Plant-based diets supplemented with PBM and H. illucens pupae meal have great potential as alternative diets for European seabass, without affecting growth performance, gut homeostasis, or overall fitness. This also highlights the importance of animal proteins in diets of European seabass, as the addition of a small amount of these alternative animal protein sources significantly improved all measured parameters.

Ivana Lepen Pleić;Ivana Bušelić;Maria Messina;Jerko Hrabar;LukaŽuvić;Igor Talijančić;IvaŽužul;Tina Pavelin;Ivana Anđelić;Jelka Pleadin;Jasna Puizina;Leon Grubišić;Emilio Tibaldi;TanjaŠegvić-Bubić

Laboratory of Aquaculture,Institute of Oceanography and Fisheries,?etali?te I.Me?trovi?a 63,21000 Split,Croatia;Department of Agriculture,Food, Environmental and Animal Sciences,University of Udine,Udine,Italy;Department of Chemistry,Faculty of Science,University of Split,Ru?era Bo?kovi?a 33,21000 Split,Croatia;Croatian Veterinary Institute,Laboratory for Analytical Chemistry,Savska Cesta 143,10000,Zagreb,Croatia;Department of Biology,Faculty of Science,University of Split,Ru?era Bo?kovi?a 33,21000 Split,Croatia

畜牧与生物技术杂志（英文版）

[1]Ivana Lepen Pleić;Ivana Bušelić;Maria Messina;Jerko Hrabar;LukaŽuvić;Igor Talijančić;IvaŽužul;Tina Pavelin;Ivana Anđelić;Jelka Pleadin;Jasna Puizina;Leon Grubišić;Emilio Tibaldi;TanjaŠegvić-Bubić-.A plant-based diet supplemented with Hermetia illucens alone or in combination with poultry by-product meal:one step closer to sustainable aquafeeds for European seabass)[J].畜牧与生物技术杂志（英文版）,2022(06):1818-1839

aquafeeds,seabass,VH10,VH10P30,FH10,subadult,Expectedly

plant,supplemented,Hermetia,illucens,alone,combination,poultry,by,product,step,closer,sustainable,European,Background,Increasing,demand,value,species,pressure,forage,challenging,aquaculture,ensure,growth,replacing,sources,terrestrial,animal,proteins,without,compromising,economic,quality,final,present,study,effects,CV,two,diets,which,graded,amounts,mixtures,were,replaced,PBM,fishmeal,FM,CF,performance,gut,health,homeostasis,farmed,tested,compared,Results,Fish,fed,showed,highest,specific,rates,lowest,conversion,ratios,among,groups,best,preservation,morphology,was,observed,while,exhibited,degenerative,changes,proximal,distal,intestines,However,supplementation,mitigated,these,significantly,improved,morphometric,parameters,Partial,substitution,insect,also,induced,most,BBM,genes,activated,enzymes,suggesting,beneficial,intestinal,digestive,absorption,functions,Regarding,microbiota,containing,had,richness,bacterial,communities,abundance,such,Lactobacillus,Bacillus,On,other,hand,microbial,diversity,but,lost,component,phylum,Bacteroidetes,Finally,skin,pigmentation,similar,that,even,wild,Conclusion,Plant,pupae,have,great,potential,alternative,affecting,overall,fitness,This,highlights,importance,addition,small,measured

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