Due to their special anatomical and physiological features,central nervous system diseases still presented challenges,despite the fact that some advances have been made in early diagnosis and precision medicine.One of the complexities in treating tumors is the tumor microenvironment,which includes mesenchymal stem cells(MSCs)that exhibit tumor tropism and can be used for cell therapy.However,whether MSCs promote or suppress gliomas is still unclear,especially in glioma microenvironments.In this study,a coaxial microfiber was designed to mimic the tumor microenvironment and to reveal the effect of MSCs on glioma cells.The fiber shell was composed of MSCs and alginate,and the core was filled with U87MG(glioblastoma)cells and gelatin methacrylate.This Shell-MSC/Core-U87MG microenvironment improved the prolifera-tion,survival,invasion,metastasis,and drug resistance of glioma cells,while simultaneously maintaining the sternness of glioma cells.In summary,coaxial extrusion bioprinted Shell-MSC/Core-U87MG microfiber is an ideal platform for tumor and stromal cell coculture to observe tumor biological behavior in vitro.

Zhizhong Jin;Xinda Li;Boxun Liu;Xinru Yan;Shuai Han;Tao Xu;Anhua Wu

Department of Neurosurgery,The First Hospital of China Medical University,Shenyang 110001,China;Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing,Department of Mechanical Engineering,Tsinghua University,Beijing 100084,China;Department of Precision Medicine and Healthcare,Tsinghua-Berkeley Shenzhen Institute,Shenzhen 518055,China;East China Institute of Digital Medical Engineering,Shangrao 334000,China

生物设计与制造（英文）

[1]Zhizhong Jin;Xinda Li;Boxun Liu;Xinru Yan;Shuai Han;Tao Xu;Anhua Wu-.Coaxial bioprinted microfibers with mesenchymal stem cells for glioma microenvironment simulation)[J].生物设计与制造（英文）,2022(02):348-357

bioprinted,sternness

Coaxial,microfibers,mesenchymal,cells,simulation,Due,their,anatomical,physiological,features,central,nervous,system,diseases,still,presented,challenges,despite,fact,that,some,advances,have,been,made,early,diagnosis,precision,medicine,One,complexities,treating,tumors,which,includes,MSCs,exhibit,tropism,can,used,therapy,However,whether,promote,suppress,gliomas,unclear,especially,microenvironments,In,this,study,coaxial,was,designed,mimic,reveal,effect,shell,composed,alginate,core,filled,U87MG,glioblastoma,gelatin,methacrylate,This,Shell,Core,improved,prolifera,survival,invasion,metastasis,drug,resistance,while,simultaneously,maintaining,summary,extrusion,ideal,platform,stromal,coculture,observe,biological,behavior,vitro

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