Current electrospun membranes used for pulp capping still lack the sustained-release capability and long-term anti-inflamma-tory effects that are favorable for dental pulp regeneration.In this work,a single-layered poly(lactic acid)(PLA)electrospun membrane loaded with amorphous calcium phosphate(ACP)and aspirin(PLA/ACP/Aspirin membrane,i.e.,PAA membrane)is sandwiched between two poly(lactic-co-glycolic acid)(PLGA)electrospun membranes as a novel sandwich-structured PLGA and PAA composite electrospun membrane(PLGA-PAA membrane)to resolve the need for sustained-release design and anti-inflammatory effects.Contact angle measurements indicate that the PLGA-PAA membrane is more hydrophilic than the PAA membrane.An in vitro release study reveals that PLGA membranes coated on PAA membrane could slightly slow down ion release,while significantly prolonging aspirin release.We also co-cultured membranes with dental pulp stem cells(DPSCs)and human monocytic THP-1 cells to evaluate their osteogenic ability and anti-inflammatory effects,respectively.Compared with the PAA membrane,the PLGA-PAA membrane promotes cell adhesion,proliferation,and osteogenic differentiation.A prolonged anti-inflammatory effect of up to 18 days is also observed in the PLGA-PAA group.The results suggest a promising strategy for fabricating an electrospun membrane system with controlled release capabili-ties and long-term anti-inflammatory effects for use as pulp-capping material for regeneration of the dentin-pulp complex.

Fenghe Yang;Jiangxue Wang;Xiaoyu Li;Zhenzhen Jia;Qiang Wang;Dazhi Yu;Jinyu Li;Xufeng Niu

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education,Beijing Advanced Innovation Center for Biomedical Engineering,School of Biological Science and Medical Engineering,Beihang University,Beijing 100083,China;Department of Burn Plastic Surgery,80th Army Hospital of PLA,Weifang 261021,China;Department of Hand Surgery,971 st Army Hospital of PLA,Qingdao 266000,China;Department of Orthopaedics,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China

生物设计与制造（英文）

[1]Fenghe Yang;Jiangxue Wang;Xiaoyu Li;Zhenzhen Jia;Qiang Wang;Dazhi Yu;Jinyu Li;Xufeng Niu-.Electrospinning of a sandwich-structured membrane with sustained release capability and long-term anti-inflammatory effects for dental pulp regeneration)[J].生物设计与制造（英文）,2022(02):305-317

monocytic,capabili

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