典型文献
Time-course monitoring of in vitro biotransformation reaction via solid-phase microextraction-ambient mass spectrometry approaches
文献摘要：
The solid-phase microextraction technique quantifies analytes without considerably affecting the sample composition.Herein,a proof-of-concept study was conducted to demonstrate the use of coated probe electrospray ionization(coated-PESI)and coated blade spray(CBS)as ambient mass spectrometry ap-proaches for monitoring drug biotransformation.The ability of these methods was investigated for monitoring the dephosphorylation of a prodrug,combretastatin A4 phosphate(CA4P),into its active form,combretastatin A4(CA4),in a cell culture medium supplemented with fetal bovine serum.The CBS spot analysis was modified to achieve the same extraction efficiency as protein precipitation and ob-tained results in 7 min.Because coated-PESI performs extraction without consuming any samples,it is the preferred technique in the case of a limited sample volume.Although coated-PESI only extracts small quantities of analytes,it uses the desorption solvent volume of 5-10 pL,resulting in high sensitivity,thus allowing the detection of compounds after only 1 min of extraction.The biotransformation of CA4P into CA4 via phosphatases occurs within the simple matrix,and the proposed sample preparation techniques are suitable for monitoring the biotransformation.
文献关键词：
中图分类号：
作者姓名：
Karol Jaroch;Janusz Pawliszyn
作者机构：
Department of Chemistry,University of Waterloo,Waterloo,N2L 3G1,Canada;Department of Pharmacodynamics and Molecular Pharmacology,Faculty of Pharmacy,Collegium Medicum in Bydgoszcz,Nicolaus Copernicus University in Torun Poland,85090,Bydgoszcz,Poland
文献出处：
引用格式：
[1]Karol Jaroch;Janusz Pawliszyn-.Time-course monitoring of in vitro biotransformation reaction via solid-phase microextraction-ambient mass spectrometry approaches)[J].药物分析学报（英文）,2022(01):186-191
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Time,course,monitoring,vitro,biotransformation,reaction,via,solid,phase,microextraction,ambient,mass,spectrometry,approaches,quantifies,analytes,without,considerably,affecting,composition,Herein,proof,concept,study,was,conducted,demonstrate,coated,probe,electrospray,ionization,PESI,blade,CBS,ability,these,methods,investigated,dephosphorylation,prodrug,combretastatin,phosphate,CA4P,into,its,active,cell,culture,medium,supplemented,fetal,bovine,serum,spot,analysis,modified,achieve,same,efficiency,protein,precipitation,tained,results,Because,performs,consuming,any,samples,preferred,case,limited,volume,Although,only,extracts,small,quantities,uses,desorption,solvent,pL,resulting,high,sensitivity,thus,allowing,detection,compounds,after,phosphatases,occurs,within,simple,matrix,proposed,preparation,techniques,are,suitable
AB值：
0.574893
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