Objective:Epidemiology studies indicate that green tea polyphenols(GTP)perform a protective effect on cardiovascular diseases,but the underlying mechanisms are complex.The present study aimed to investigate the effect of GTP on high-fat diets(HFD)induced-early vascular aging.Methods:Six-week young adult Wistar rats were fed with standard chow or HFD in the presence and absence of GTP(200 mg/kg body weight)for 18 weeks.In vitro experiment,human umbilical vascular endothelial cells(HUVECs)were treated with palmitic acid(PA)and GTP.Results:The results showed that GTP alleviated the disorganized arterial wall and the increased intima-media thickness induced by HFD.In addition,the vascular oxidative injury was suppressed following GTP treatment.Furthermore,GTP elevated the ratio of LC3-II/LC3-I and suppressed expression of p62/SQSTM1,and restored SIRT3 expression in the aorta of HFD rats.Consistently,in cultured HUVECs,GTP inhibited cell senescence indicated by SA-p-gal and promoted endothelial autophagy compared with the PA treatment group.The activity of SIRT3 was specifically inhibited by 3-TYP,and the protective effect of GTP was consequently abolished.Conclusion:The findings indicated that GTP protected against early vascular senescence in young HFD rats via ameliorating oxidative injury and promoting autophagy which was partially regulated by the SIRT3 pathway.

Xiang-tian XIAO;Shui-qing HE;Nan-nan WU;Xue-chun LIN;Jing ZHAO;Chong TIAN

Medical College of Wuhan University of Science and Technology,Wuhan 430081,China;Department of Nutrition and Food Hygiene,Hubei Key Laboratory of Food Nutrition and Safety,School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;School of Public Health and Management,Research Center for Medicine and Social Development,Innovation Center for Social Risk Governance in Health,Chongqing Medical University,Chongqing 400016,China;School of Nursing,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

当代医学科学(英文)

[1]Xiang-tian XIAO;Shui-qing HE;Nan-nan WU;Xue-chun LIN;Jing ZHAO;Chong TIAN-.Green Tea Polyphenols Prevent Early Vascular Aging Induced by High-Fat Diet via Promoting Autophagy in Young Adult Rats)[J].当代医学科学(英文),2022(05):981-990

disorganized

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