典型文献
Xuebijing Injection Ameliorates H2S-Induced Acute Respiratory Distress Syndrome by Promoting Claudin-5 Expression
文献摘要：
Objective:To investigate the protective effects and underlying mechanisms of Xuebijing Injection (XBJ) on the lung endothelial barrier in hydrogen sulfide (H2S)-induced acute respiratory distress syndrome (ARDS).Methods:Sprague-Dawley rats were exposed to H2S (300 ppm) to establish ARDS model,while human pulmonary microvascular endothelial cells (HPMECs) were incubated with NaHS(a H2S donor,500 μ mol/L) to establish cell model.H2S and XBJ were concurrently administered to the rat and cell models.Lung hematoxylin and eosin staining,immunohistochemistry,transmission electron microscopy and wet/dry ratio measurement were used to confirm ARDS induced by H2S in vivo.The expression levels of claudin-5,phosphorylated protein kinase B (p-AKT)/t-AKT and p-forkhead box transcription factor O1(FoxO1)/t-FoxO1 in vivo and in vitro were also assessed.Paracellular permeability and transepithelial electrical resistance (TEER) were measured to evaluate endothelial barrier function in the cell model.Results:The morphological investigation showed that XBJ attenuated H2S-induced ARDS in rats.XBJ significantly ameliorated both the reduction in TEER and the increased paracellular permeability observed in NaHS-treated HPMECs (P＜0.05).The protective effects of XBJ were blocked by LY294002,a phosphatidylinositol 3-kinase(PI3K)/AKT/FoxO1 pathway antagonist (P＜0.05).Furthermore,XBJ promoted the expression of claudin-5 and increased the levels of p-AKT and p-FoxO1 in vivo and in vitro (P＜0.05).Conclusion:XBJ ameliorated H2S-induced ARDS by promoting claudin-5 expression via the PI3K/AKT/FoxO1 signaling pathway.
文献关键词：
中图分类号：
作者姓名：
GENG Ping;LING Bing-yu;ZHANG Hong-liang;XIONG Jia-li;WANG Ying;YU Fen;TAN Ding-yu;XU Ji-yang;WANG Hui-hui
作者机构：
Department of Emergency,Clinical Medical College of Yangzhou University,Northern Jiangsu People's Hospital,Yangzhou,Jiangsu Province 225001 ,China;The First Clinical Medical College of Dalian Medical University,Dalian,Liaoning Province 116044 ,China
文献出处：
引用格式：
[1]GENG Ping;LING Bing-yu;ZHANG Hong-liang;XIONG Jia-li;WANG Ying;YU Fen;TAN Ding-yu;XU Ji-yang;WANG Hui-hui-.Xuebijing Injection Ameliorates H2S-Induced Acute Respiratory Distress Syndrome by Promoting Claudin-5 Expression)[J].中国结合医学杂志（英文版）,2022(02):116-123
A类：
Paracellular
B类：
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AB值：
0.553164
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