Objective This study aimed to investigate the effects of caprylic acid (C8:0) on lipid metabolism and inflammation, and examine the mechanisms underlying these effects in mice and cells. Methods Fifty-six 6-week-old male C57BL/6J mice were randomly allocated to four groups fed a high-fat diet (HFD) without or with 2％ C8:0, palmitic acid (C16:0) or eicosapentaenoic acid (EPA). RAW246.7 cells were randomly divided into five groups: normal, lipopolysaccharide (LPS), LPS+C8:0, LPS+EPA and LPS+cAMP. The serum lipid profiles, inflammatory biomolecules, and ABCA1 and JAK2/STAT3 mRNA and protein expression were measured. Results C8:0 decreased TC and LDL-C, and increased the HDL-C/LDL-C ratio after injection of LPS. Without LPS, it decreased TC in mice (P < 0.05). Moreover, C8:0 decreased the inflammatory response after LPS treatment in both mice and cells (P < 0.05). Mechanistic investigations in C57BL/6J mouse aortas after injection of LPS indicated that C8:0 resulted in higher ABCA1 and JAK2/STAT3 expression than that with HFD, C16:0 and EPA, and resulted in lower TNF-α, NF-κB mRNA expression than that with HFD (P < 0.05). In RAW 264.7 cells, C8:0 resulted in lower expression of pNF-κBP65 than that in the LPS group, and higher protein expression of ABCA1, p-JAK2 and p-STAT3 than that in the LPS and LPS+cAMP groups (P < 0.05). Conclusion Our studies demonstrated that C8:0 may play an important role in lipid metabolism and the inflammatory response, and the mechanism may be associated with ABCA1 and the p-JAK2/p-STAT3 signaling pathway.

ZHANG Xin Sheng;ZHANG Peng;LIU Ying Hua;XU Qing;ZHANG Yong;LI Hui Zi;LIU Lu;LIU Yu Meng;YANG Xue Yan;XUE Chang Yong

Department of Nutrition,the First Medical Center of PLA General Hospital,Beijing 100853,China;Guizhou Crops of Chinese Armed Police Force,Guiyang 550001,Guizhou,China;Department of Nutrition,PLA Rocket Force Characteristic Medical Center,Beijing 100088,China

生物医学与环境科学（英文版）

[1]ZHANG Xin Sheng;ZHANG Peng;LIU Ying Hua;XU Qing;ZHANG Yong;LI Hui Zi;LIU Lu;LIU Yu Meng;YANG Xue Yan;XUE Chang Yong-.Caprylic Acid Improves Lipid Metabolism, Suppresses the Inflammatory Response and Activates the ABCA1/p-JAK2/p- STAT3 Signaling Pathway in C57BL/6J Mice and RAW264.7 Cells)[J].生物医学与环境科学（英文版）,2022(02):95-106,中插1

Caprylic,Activates,LPS+C8,LPS+EPA,LPS+cAMP

Acid,Improves,Lipid,Metabolism,Suppresses,Inflammatory,Response,ABCA1,JAK2,STAT3,Signaling,Pathway,C57BL,6J,Mice,RAW264,Cells,Objective,This,study,aimed,investigate,effects,caprylic,acid,lipid,metabolism,inflammation,examine,mechanisms,underlying,these,mice,cells,Methods,Fifty,six,week,old,male,were,randomly,allocated,four,groups,fed,fat,diet,HFD,without,palmitic,C16,eicosapentaenoic,RAW246,divided,into,five,normal,lipopolysaccharide,serum,profiles,inflammatory,biomolecules,protein,expression,measured,Results,decreased,LDL,increased,HDL,ratio,after,injection,Without,Moreover,response,treatment,both,Mechanistic,investigations,mouse,aortas,indicated,that,resulted,higher,than,lower,pNF,BP65,Conclusion,Our,studies,demonstrated,may,play,important,role,be,associated,signaling,pathway

0.477155