典型文献
Efficient generation of targeted point mutations in the Brassica oleracea var.botrytis genome via a modified CRISPR/Cas9 system
文献摘要：
In this study,we used the modified CRISPR/Cas9 system to produce targeted point mutations in cauliflower.Acetolactate synthase(ALS)and Centromere-specific histone H3 variant(CENH3)genes were selected as the base-editing targets and hypocotyls of cauliflower were used as explants.For ALS gene,a C-to-T conversion in the Pro182 codon(CCT)can alter the encoded amino acid,likely resulting in herbicide resistance,and a C-to-T mutation in the Leu133 codon(CTT)in the CENH3 gene may produce a haploid inducer.Results indicated that the transformation efficiency was 1.8％-4.5％and the mutation efficiencies for the ALS and CENH3 genes were approximately 22％and 87％,respectively.The ALS mutant cauliflower showed strong herbicide resistance,with possible immediate implications for broadleaf weed control in cauliflower fields.
文献关键词：
中图分类号：
作者姓名：
Guixiang Wang;Mei Zong;Di Liu;Yage Wu;Shouwei Tian;Shuo Han;Ning Guo;Mengmeng Duan;Liming Miao;Fan Liu
作者机构：
Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture,Beijing Key Laboratory of Vegetable Germplasm Improvement,Beijing 100097,China
文献出处：
引用格式：
[1]Guixiang Wang;Mei Zong;Di Liu;Yage Wu;Shouwei Tian;Shuo Han;Ning Guo;Mengmeng Duan;Liming Miao;Fan Liu-.Efficient generation of targeted point mutations in the Brassica oleracea var.botrytis genome via a modified CRISPR/Cas9 system)[J].园艺学报（英文版）,2022(04):527-530
A类：
Acetolactate,Centromere,Pro182,Leu133
B类：
Efficient,generation,targeted,point,mutations,Brassica,oleracea,botrytis,genome,via,modified,CRISPR,Cas9,system,In,this,study,used,produce,cauliflower,synthase,ALS,specific,histone,variant,CENH3,genes,were,selected,base,editing,targets,hypocotyls,explants,For,conversion,codon,CCT,can,alter,encoded,amino,acid,likely,resulting,herbicide,resistance,CTT,may,haploid,inducer,Results,indicated,that,transformation,efficiency,was,efficiencies,approximately,respectively,mutant,showed,strong,possible,immediate,implications,broadleaf,weed,control,fields
AB值：
0.564395
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