典型文献
Effects of sgRNA length and number on gene editing efficiency and predicted mutations generated in rice
文献摘要：
CRISPR-Cas9 is a common tool for gene editing,and appropriate sgRNAs are the key factor for successful editing.In this study,the effect of sgRNA length and number on editing efficiency was analyzed in rice using CYP81A6 as the target gene.A series of CRISPR-Cas9 plant expression vectors containing single sgRNAs with different lengths(17,18,19,20,21,22,23 nt)or two sgRNAs were constructed and intro-duced into rice cultivar Zhonghua11 by Agrobacterium-mediated transformation.Analysis of the editing status of 1283 transgenic rice plants showed that 371 were successfully edited with base preference.Single A or T insertions were the most frequent among the six edited types.The editing efficiency of transgenic rice with two sgRNAs was higher than that with a single sgRNA.Editing efficiency and sgRNA length showed a normal distribution with 20 nt sgRNA(25％)being the most efficient.The editing efficiency decreased slightly with decreases of 1-2 bases(19 nt 20％,18 nt 21％),but decreased signifi-cantly with a decrease of 3 bases(17 nt 4.5％).Editing efficiency was significantly reduced by adding 1 to 3 bases(21 nt 16.8％,22 nt 13％,23 nt 13％)to the sgRNA.These results provide data for successful gene editing or rice by CRISPR-Cas9.
文献关键词：
中图分类号：
作者姓名：
Xiaojing Liu;Jiangtao Yang;Yaya Song;Xiaochun Zhang;Xujing Wang;Zhixing Wang
作者机构：
Biotechnology Research Institute,Chinese Academy of Agricultural Sciences/MARA Key Laboratory on Safety Assessment(Molecular)of Agri-GMO,Beijing 100081,China
文献出处：
引用格式：
[1]Xiaojing Liu;Jiangtao Yang;Yaya Song;Xiaochun Zhang;Xujing Wang;Zhixing Wang-.Effects of sgRNA length and number on gene editing efficiency and predicted mutations generated in rice)[J].作物学报（英文版）,2022(02):577-581
A类：
CYP81A6,Zhonghua11
B类：
Effects,number,editing,efficiency,predicted,mutations,generated,rice,CRISPR,Cas9,common,tool,appropriate,sgRNAs,are,key,In,this,study,effect,was,analyzed,using,target,series,expression,vectors,containing,single,different,lengths,two,were,constructed,intro,into,cultivar,by,Agrobacterium,mediated,transformation,Analysis,status,transgenic,plants,showed,that,successfully,edited,preference,Single,insertions,most,frequent,among,six,types,higher,than,Editing,normal,distribution,being,efficient,decreased,slightly,decreases,bases,significantly,reduced,adding,These,results,provide,data
AB值：
0.410834
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
