典型文献
Development of mutants with varying flowering times by targeted editing of multiple SVP gene copies in Brassica napus L.
文献摘要：
Manipulation of flowering time to develop cultivars with desired maturity dates is fundamental in plant breeding. It is desirable to generate polyploid rapeseed (Brassica napus L.) germplasm with varying flow-ering time controlled by a few genes. In the present study, BnaSVP, a rapeseed homolog of the Arabidopsis SVP (Short Vegetative Phase) gene, was characterized and a set of mutants was developed using a CRISPR/Cas9-based gene-editing tool. A single construct targeting multiple sites was successfully applied to pre-cisely mutate four copies of BnaSVP. The induced mutations in these copies were stably transmitted to subsequent generations. Homozygous mutants with loss-of-function alleles and free transgenic elements were generated across the four BnaSVP homologs. All mutant T1 lines tested in two environments (sum-mer and winter growing seasons) showed early-flowering phenotypes. The decrease in flowering time was correlated with the number of mutated BnaSVP alleles. The quadruple mutants showed the shortest flowering time, with a mean decrease of 40.6％–50.7％in length relative to the wild type under the two growth conditions. Our study demonstrates the quantitative involvement of BnaSVP copies in the regu-lation of flowering time and provides valuable resources for rapeseed breeding.
文献关键词：
中图分类号：
作者姓名：
Sunny Ahmar;Yungu Zhai;Huibin Huang;Kaidi Yu;Muhammad Hafeez Ullah Khan;Muhammad Shahid;Rana Abdul Samad;Shahid Ullah Khan;Olalekan Amoo;Chuchuan Fan;Yongming Zhou
作者机构：
National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,Hubei,China
文献出处：
引用格式：
[1]Sunny Ahmar;Yungu Zhai;Huibin Huang;Kaidi Yu;Muhammad Hafeez Ullah Khan;Muhammad Shahid;Rana Abdul Samad;Shahid Ullah Khan;Olalekan Amoo;Chuchuan Fan;Yongming Zhou-.Development of mutants with varying flowering times by targeted editing of multiple SVP gene copies in Brassica napus L.)[J].作物学报（英文版）,2022(01):67-74
A类：
BnaSVP,Vegetative,mutate,Homozygous
B类：
Development,mutants,varying,flowering,times,by,targeted,editing,multiple,copies,Brassica,napus,Manipulation,cultivars,desired,maturity,dates,fundamental,plant,breeding,It,desirable,polyploid,rapeseed,germplasm,controlled,few,genes,In,present,study,Arabidopsis,Short,Phase,was,characterized,set,developed,using,CRISPR,Cas9,tool,single,construct,targeting,sites,successfully,applied,cisely,four,induced,mutations,these,were,stably,transmitted,subsequent,generations,loss,function,alleles,free,transgenic,elements,generated,across,homologs,All,lines,tested,two,environments,sum,mer,winter,growing,seasons,showed,early,phenotypes,decrease,correlated,number,mutated,quadruple,shortest,mean,length,relative,wild,under,growth,conditions,Our,demonstrates,quantitative,involvement,regu,provides,valuable,resources
AB值：
0.532506
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
