Viruses cause many severe diseases in both plants and animals, urging us to explore new an-tiviral strategies. In their natural reservoirs, viruses live and replicate while causing mild or no symp-toms. Some animals, such as bats, are the predicted natural reservoir of multiple viruses, in-dicating that they possess broad-spectrum antiviral capabilities. Mechanisms of host de-fenses against viruses are generally studied in-dependently in plants and animals. In this article, we speculate that some antiviral strategies of natural reservoirs are conserved between king-doms. To verify this hypothesis, we created null mutants of 10-formyltetrahydrofolate synthetase (AtTHFS), an Arabidopsis thaliana homologue of methylenetetrahydrofolate dehydrogenase, cyclo-hydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), which encodes a positive regulator of viral replication in bats. We found that disruption of AtTHFS enhanced plant resistance to three different types of plant viruses, including the to-mato spotted wilt virus (TSWV), the cucumber mosaic virus (CMV) and the beet severe curly top virus (BSCTV). These results demonstrate a novel antiviral strategy for plant breeding. We further discuss the approaches used to identify and study natural reservoirs of plant viruses, especially those hosting many viruses, and highlight the possibility of discovering new antiviral strategies from them for plant molecular breeding and antiviral therapy.

Peking University Institute of Advanced Agricultural Sciences,Weifang 261325,China;The Key Laboratory of Plant Development and Environmental Adaptation Biology,Ministry of Education,School of Life Sciences,Shandong University,Qingdao266237,China

[1]Wenbo Pan;Weiwei Li;Lijing Liu;Huawei Zhang-.Antiviral strategies:What can we learn from natural reservoirs?FA)[J].植物学报（英文版）,2022(10):1849-1855

urging,tiviral,fenses,doms,formyltetrahydrofolate,AtTHFS,mato,BSCTV

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