典型文献
A new anterograde trans-synaptic tracer based on Sindbis virus
文献摘要：
Mapping neural circuits is critical for understanding the structure and function of the nervous system.Engineered viruses are a valuable tool fortracing neural circuits.However,current tracers do not fully meet the needs for this approach because of various drawbacks,such as toxicity and characteristics that are difficult to modify.Therefore,there is an urgent need to develop a new tracer with low toxicity and that allows for long-term studies.In this study,we constructed an engineered Sindbis virus(SINV)expressing enhanced green fluorescent protein(EGFP)reporter gene(SINV-EGFP)and found that it had no significant difference in biological characterization compared with the wild-type Sindbis virus in BHK-21 cells and neurons in vitro.We injected the virus into the visual circuit of mouse brain and found that the virus infected neurons in the local injected site and anterogradely spread in the neural circuits.Although the efficiency of transmission was limited,the findings demonstrate that SINV can be used as a new anterograde tracer to map neural circuits in mouse brain and that it spreads exclusively in the anterograde direction.Further,use of SINV in mouse brain research will provide longer time windows for circuit tracing than is possible with herpes simplex virus and vesicular stomatitis virus tracers.
文献关键词：
中图分类号：
作者姓名：
Xiang-Wei Shi;Fan Jia;Pei Lyu;Fu-Qiang Xu
作者机构：
State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,Key Laboratory of Magnetic Resonance in Biological Systems,Wuhan Center for Magnetic Resonance,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences,Wuhan,Hubei Province,China;Guangdong Provincial Key Laboratory of Brain Connectome and Behavior,CAS Key Laboratory of Brain Connectome and Manipulation,the Brain Cognition and Brain Disease Institute(BCBDI),Translational Research Center for the Nervous System(TRCNS),Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences;Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions,Shenzhen,Guangdong Province,China;NMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal Products,Key Laboratory of Quality Control Technology for Virus-Based Therapeutics,Guangdong Provincial Medical Products Administration,Shenzhen Key Laboratory of Viral Vectors for Biomedicine,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong Province,China;University of Chinese Academy of Sciences,Beijing,China;University of Chinese Academy of Sciences,Beijing,China4;Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei Province, China
文献出处：
引用格式：
[1]Xiang-Wei Shi;Fan Jia;Pei Lyu;Fu-Qiang Xu-.A new anterograde trans-synaptic tracer based on Sindbis virus)[J].中国神经再生研究（英文版）,2022(12):2761-2764
A类：
anterograde,fortracing,SINV,anterogradely
B类：
new,synaptic,Sindbis,Mapping,neural,circuits,critical,understanding,structure,function,nervous,system,Engineered,viruses,valuable,tool,However,current,tracers,not,fully,meet,needs,this,approach,because,various,drawbacks,such,toxicity,characteristics,that,difficult,modify,Therefore,there,urgent,develop,allows,term,studies,In,study,constructed,engineered,expressing,enhanced,green,fluorescent,protein,EGFP,reporter,gene,found,had,significant,difference,biological,characterization,compared,wild,type,BHK,cells,neurons,vitro,We,injected,into,visual,mouse,brain,infected,local,site,Although,efficiency,transmission,was,limited,findings,demonstrate,used,map,spreads,exclusively,direction,Further,research,will,provide,longer,windows,than,possible,herpes,simplex,vesicular,stomatitis
AB值：
0.500859
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