Transcriptomic studies are an important tool for understanding the molecular pathways underlying host plant use by agricultural pests,including vectors of damaging plant pathogens.Thus far,bulk RNA-Seq has been the main approach for non-model in-sects.This method relies on pooling large numbers of whole organisms or hundreds of in-dividually dissected organs.The latter approach is logistically challenging,may introduce artifacts of handling and storage,and is not compatible with biological replication.Here,we tested an approach to generate transcriptomes of individual salivary glands and other low-input body tissues from whiteflies(Bemisia tabaci MEAM1),which are major vectors of plant viruses.By comparing our outputs to published bulk RNA-Seq datasets for whole whitefly bodies and pools of salivary glands,we demonstrate that this approach recov-ers similar numbers of transcripts relative to bulk RNA-Seq in a tissue-specific manner,and for some metrics,exceeds performance of bulk tissue RNA-Seq.Libraries generated from individual salivary glands also yielded additional novel transcripts not identified in pooled salivary gland datasets,and had hundreds of enriched transcripts when compared with whole head tissues.Overall,our study demonstrates that it is feasible to produce high quality,replicated transcriptomes of whitefly salivary glands and other low-input tissues.We anticipate that our approach will expand hypothesis-driven research on salivary glands of whiteflies and other Hemiptera,thus enabling novel control strategies to disrupt feeding and virus transmission.

Marco Gebiola;Brandon H.Le;Kerry E.Mauck

Department of Entomology,University of California Riverside,Riverside,California;Department of Botany and Plant Sciences,University of California Riverside,Riverside,California;Institute of Integrative Genome Biology,University of California Riverside,Riverside,California,USA

中国昆虫科学（英文版）

[1]Marco Gebiola;Brandon H.Le;Kerry E.Mauck-.A reproducible and sensitive method for generating high-quality transcriptomes from single whitefly salivary glands and other low-input tissues)[J].中国昆虫科学（英文版）,2022(05):1318-1328

whitefly,sects,dividually,logistically,whiteflies

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