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Natural variation in the NAC transcription factor NONRIPENING contributes to melon fruit ripening
文献摘要:
The NAC transcription factor NONRIPENING (NOR) is a master regulator of climacteric fruit ripening. Melon (Cucumis melo L.) has climacteric and non-climacteric fruit ripening varieties and is an ideal model to study fruit ripening. Two natural CmNAC-NOR variants, the climacteric haplotype CmNAC-NORS,N and the non-climacteric haplotype CmNAC-NORA,S, have effects on fruit ripening;how-ever, their regulatory mechanisms have not been elucidated. Here, we report that a natural mutation in the transcriptional activation domain of CmNAC- NORS,N contributes to climacteric melon fruit rip-ening. CmNAC-NOR knockout in the climacteric-type melon cultivar"BYJH"completely inhibited fruit rip-ening, while ripening was delayed by 5–8 d in heter-ozygous cmnac-nor mutant fruits. CmNAC-NOR di-rectly activated carotenoid, ethylene, and abscisic acid biosynthetic genes to promote fruit coloration and ripening. Furthermore, CmNAC-NOR mediated the transcription of the"CmNAC-NOR-CmNAC73-CmCWINV2"module to enhance flesh sweetness. The transcriptional activation activity of the climac-teric haplotype CmNAC-NORS,N on these target genes was significantly higher than that of the non-climacteric haplotype CmNAC-NORA,S. Moreover, CmNAC-NORS,N complementation fully rescued the non-ripening phenotype of the tomato (Solanum ly-copersicum) cr-nor mutant, while CmNAC-NORA,S did not. Our results provide insight into the molecular mechanism of climacteric and non-climacteric fruit ripening in melon.
文献关键词:
作者姓名:
Jinfang Wang;Shouwei Tian;Yongtao Yu;Yi Ren;Shaogui Guo;Jie Zhang;Maoying Li;Haiying Zhang;Guoyi Gong;Min Wang;Yong Xu
作者机构:
National Watermelon and Melon Improvement Center,Beijing Academy of Agricultural and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Beijing Key Laboratory of Vegetable Germplasm Improvement,Beijing 100097,China;Sanya Institute,Hainan Academy of Agricultural Sciences,Haikou 572025,China
引用格式:
[1]Jinfang Wang;Shouwei Tian;Yongtao Yu;Yi Ren;Shaogui Guo;Jie Zhang;Maoying Li;Haiying Zhang;Guoyi Gong;Min Wang;Yong Xu-.Natural variation in the NAC transcription factor NONRIPENING contributes to melon fruit ripening)[J].植物学报(英文版),2022(07):1448-1461
A类:
NONRIPENING,climacteric,CmNAC,BYJH,ozygous,cmnac,CmNAC73,CmCWINV2,sweetness,climac,teric,copersicum
B类:
Natural,variation,contributes,melon,ripening,master,Melon,Cucumis,has,varieties,ideal,model,study,Two,natural,variants,haplotype,NORS,NORA,have,effects,how,ever,their,regulatory,mechanisms,been,elucidated,Here,report,that,mutation,transcriptional,activation,domain,knockout,cultivar,completely,inhibited,while,was,delayed,by,heter,nor,mutant,fruits,rectly,activated,carotenoid,ethylene,abscisic,acid,biosynthetic,genes,promote,coloration,Furthermore,mediated,module,enhance,flesh,activity,these,target,significantly,higher,than,Moreover,complementation,fully,rescued,phenotype,tomato,Solanum,did,Our,results,provide,insight,into,molecular
AB值:
0.340779
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