典型文献
Overexpression of the apple expansin-like gene MdEXLB1 accelerates the softening of fruit texture in tomato
文献摘要：
Fruit firmness is an important quality trait of apple fruit texture,and the pre-harvest ripening period is the key period for the formation of apple fruit texture.Expansin is a cell wall loosing protein family that has four subfamilies:a-expansin(EXPA),β-expansin(EXPB),expansin-like A(EXLA),and expansin-like B(EXLB).In this study,we investigated the key period of pre-harvest texture formation in'Golden Delicious'apples based on fruit longitudinal diameter,transverse diameter,firmness,tissue structure,respiration intensity,ethylene release rate,and expansin activity.Within the 10 days before harvest,the fruit was found to reach maturity.Semi-quantitative RT-PCR revealed that most of the expansins were expressed at the ripening stage before harvest.The biological function of the EXLB subfamily gene,MdEXLB1,was further identified,and its subcellular localization on the cell wall was confirmed by transient transformation experiments.Compared with the wild type(WT),the transgenic tomato lines overexpressing MdEXLB1 had lower plant height,earlier fruiting period,fewer days for fruit ripening,higher fruit maturity,lower fruit firmness,higher fruit expansin activity,more discrete flesh cell structure,and accelerated fruit ripening process.Overall,this is the first study to propose that the apple EXLB subfamily gene,MdEXLB1,has biological functions and plays an important role in promoting fruit ripening and softening.
文献关键词：
中图分类号：
作者姓名：
CHEN Yan-hui;XIE Bin;AN Xiu-hong;MA Ren-peng;ZHAO De-ying;CHENG Cun-gang;LI En-mao;ZHOU Jiang-tao;KANG Guo-dong;ZHANG Yan-zhen
作者机构：
Key Laboratory of Mineral Nutrition and Efficient Fertilization for Deciduous Fruits,Liaoning Province/Key Laboratory of Fruit Germplasm Resources Utilization,Ministry of Agriculture and Rural Afffairs/Institute of Pomology,Chinese Academy of Agricultrual Sciences,Xingcheng 125100,P.R.China;National Engineering Research Center for Agriculture in Northern Mountainous Areas/Mountainous Area Research Institute,Hebei Agricultural University,Baoding 071001,P.R.China
文献出处：
引用格式：
[1]CHEN Yan-hui;XIE Bin;AN Xiu-hong;MA Ren-peng;ZHAO De-ying;CHENG Cun-gang;LI En-mao;ZHOU Jiang-tao;KANG Guo-dong;ZHANG Yan-zhen-.Overexpression of the apple expansin-like gene MdEXLB1 accelerates the softening of fruit texture in tomato)[J].农业科学学报（英文）,2022(12):3578-3588
A类：
MdEXLB1,Expansin,loosing,EXPA,EXPB,EXLA,EXLB,expansins
B类：
Overexpression,like,gene,accelerates,softening,texture,tomato,Fruit,firmness,important,quality,trait,harvest,ripening,period,key,wall,protein,that,has,four,subfamilies,In,this,study,investigated,Golden,Delicious,apples,longitudinal,diameter,transverse,tissue,structure,respiration,intensity,ethylene,release,activity,Within,days,before,was,found,reach,maturity,Semi,quantitative,revealed,most,were,expressed,stage,biological,subfamily,further,identified,its,subcellular,localization,confirmed,by,transient,transformation,experiments,Compared,wild,type,WT,transgenic,lines,overexpressing,had,lower,plant,height,earlier,fruiting,fewer,higher,more,discrete,flesh,accelerated,process,Overall,first,propose,functions,plays,role,promoting
AB值：
0.410566
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