典型文献
Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon
文献摘要：
The flesh color of oriental melons is an important commercial trait that affects consumer preferences.To explore the mechanisms un-derlying the flesh color formation and regulation during fruit ripening,carotenoid-targeted metabolomic and RNA-seq analysis were conducted between white-fleshed(WF)and orange-fleshed(OF)oriental melon cultivars at different stages.The carotenoid-targeted metabolomic analysis indicated that β-carotene was the major metabolite that caused differences in flesh color between the two cultivars.Additionally,through KEGG pathway enrichment and weighted gene co-expression network(WGCNA)analysis,metabolic pathways and related transcription factors that are associated with carotenoid metabolism were selected and transcriptome data was verified using RT-qPCR.Finally,the yeast one hybrid and luciferase activity showed that the transcription factor CmWRKY49 could directly bind to the CmPSY1 promoter to activate its expression in the'OF'cultivar.Transient overexpression of CmWRKY49 in'OF'cultivar increased the β-carotene content,while thep-carotene content decreased when it was silenced in the same cultivar.This study provides insights into the underlying regulatory network of carotenoid metabolism in oriental melon fruit.
文献关键词：
中图分类号：
作者姓名：
Xiaoyu Duan;Cai Jiang;Yaping Zhao;Ge Gao;Meng Li;Hongyan Qi
作者机构：
Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province,College of Horticulture,Shenyang Agricultural University,National and Local Joint Engineering Research Centre of Northern Horticultural,Facilities Design and Application Technology(Liaoning),Shenyang 110866,China;Nanodigmbio Biotechnology Co.,Ltd.Nanjing 210038,China
文献出处：
引用格式：
[1]Xiaoyu Duan;Cai Jiang;Yaping Zhao;Ge Gao;Meng Li;Hongyan Qi-.Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon)[J].园艺学报（英文版）,2022(05):650-666
A类：
CmWRKY49,CmPSY1,fleshed,thep
B类：
Transcriptome,metabolomics,analysis,revealed,that,regulating,promotes,carotene,accumulation,orange,oriental,color,melons,important,commercial,trait,affects,consumer,preferences,To,explore,mechanisms,formation,regulation,during,fruit,ripening,carotenoid,targeted,seq,were,conducted,between,white,WF,OF,cultivars,different,stages,indicated,was,major,metabolite,caused,differences,Additionally,through,enrichment,weighted,gene,network,WGCNA,metabolic,pathways,related,transcription,factors,are,associated,metabolism,selected,transcriptome,data,verified,using,qPCR,Finally,yeast,one,hybrid,luciferase,activity,showed,could,directly,bind,promoter,activate,its,Transient,overexpression,increased,content,while,decreased,when,silenced,same,This,study,provides,insights,into,underlying,regulatory
AB值：
0.487463
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